红掌组培快繁过程中污染原因的分析及对策探讨.doc

红掌组培快繁过程中污染原因的分析及对策探讨

摘 要

红掌（拉丁名：Spathiphyllum floribundum），亦叫安祖花，红鹤芋等，天南星科火烛属。其花色艳丽，花茎挺拔，叶型翠绿美观，是当今世界著名的切花和盆栽花卉之一，市场潜力大，具有极高的观赏价值和经济价值。红掌的繁殖方法通常采用分株繁殖、扦插繁殖和组织培养繁殖。但分株繁殖、扦插繁殖很难满足当前市场的需求，组织培养是红掌快速繁殖的有效途径。由于组织培养中经常出现不同程度的污染问题，造成很大的损失，因此控制污染是植物组织培养苗工厂化生产中重要的技术环节。本文从灭菌方法、内源性污染、继代培养中污染防治等方面，进行了红掌组培快繁过程中污染原因的分析及对策的探讨。

关键词：红掌，组织培养，灭菌，污染，防治
 
ANDRAEANUM DURING TISSUE CULTURE ANALYSIS OF THE CAUSES OF POLLUTION AND RELATED COUNTERMEASURES

ABSTRACT

Anthurium, also called Andrew Flower, lines such as flamingo, a Fire Araceae. Its bright colors, tall and straight stem, green leaf-type appearance, are well-known in today's world of cut and potted flowers, one large market potential, with high ornamental value and economic value. Flower andraeanum breeding methods commonly used ramets propagation, cutting propagation and tissue cultur e propagation. However, ramets propagation, cutting propagation is difficult to meet current market demand, tissue culture andraeanum Flower Express are an effective way of breeding. Tissue culture because of the frequent occurrence of various degrees of pollution, resulting in great loss, so control of pollution are industrial plant tissue culture vaccine production technology in the important aspect. This article from the sterilization method, endogenous pollution subculture in such areas as pollution prevention, to discuss how to minimize the emergence of tissue in the pollution problems.

KEY WORDS: Anthurium，tissue culture， sterilization， pollution，prevention and treatment
 
目　录

前　言 1
第1章 培养基的灭菌 2
1.1一般培养基的灭菌 2
1.2不耐热物质的灭菌 2
第2章 外植体材料的消毒与灭菌 4
2.1外植体的消毒与灭菌 4
第3章 接种前的准备工作 5
3.1器皿、工具的灭菌 5
3.1.1器皿与金属器械的灭菌 5
3.1.2布质制品的灭菌 5
3.2培养室的灭菌 5
3.3无菌操作和无菌操作技术 5
第4章 红掌诱导与继代培养过程中的污染 7
第5章 污染的原因和预防措施 8
5.1通常污染 8
5.2内源性污染 8
5.3污染原因判断及污染苗抢救 8
5.4预防方法和措施 9
5.4.1基本方法 9
5.4.2经常检查消毒锅的灭菌质量 9
5.4.3检查培养容器是否存在问题 10
5.4.4继代培养中污染的控制 10
5.4.5减少培养基中的有机成分 10
结　论 11
谢  辞 12
参考文献 13
附　录 15
外文资料翻译 18