头孢拉定化学稳定性研究.rar
头孢拉定化学稳定性研究,合格作品 字数3795个字摘 要头孢拉定为第一代头孢菌素类抗生素，广泛用于尿路及呼吸道感染,是目前临床应用最广泛的抗生素之一。然而，由于头孢拉定化学性质不稳定，严重地影响产品质量，甚至于影响到用药安全。本文应用ir、uv-vis、ms、hplc、xrd等检测手段，采用实验、检测与理论分析相结合的方法，从光谱与结构性质、...
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原文档由会员 白痴学东西 发布
合格作品 字数3795个字
摘 要
头孢拉定为第一代头孢菌素类抗生素，广泛用于尿路及呼吸道感染,是目前临床应用最广泛的抗生素之一。然而，由于头孢拉定化学性质不稳定，严重地影响产品质量，甚至于影响到用药安全。
本文应用IR、UV-Vis、MS、HPLC、XRD等检测手段，采用实验、检测与理论分析相结合的方法，从光谱与结构性质、破坏性因素的影响两个层面，研究了该化合物不稳定性的原因以及破坏性因素的影响程度。
研究表明：温度和结晶水对头孢拉定影响较大，常见的金属离子中MnCl2和CoAC2会与头孢拉定发生化学反应。紫外光照和晶体形貌对于该化合物稳定性影响不大。此外，本项研究还在质谱检测的基础上，提出了全新的头孢拉定酸碱裂解机理。
关键词： 头孢拉定 、化学稳定性、光谱分析
Abstract
Cephradine is 1st generation of cephalosporins, which is used in the treatment of urinary and respiratory infection and is one of the most often used antibiotic. Cephradine is a labile compound，which influents quality of product and even the safety in utilization of drugs.
In this paper, With the combination of theoretical analysis and experiment and Applying spectrum techniques，such as IR, UV-Vis, MS, HPLC, XRD to Analyzing the instability mechanism and extent of destructive factors from spectral and structural properties and disruptive factors.
Research shows that: the temperature and the crystal water greater impact on cefradine, MnCl2 and CoAC2 have obviously complex reaction with Cephradine, UV irradiation and the crystal morphology has little effect for the stability of the compound. In addition, the study proposes a new fragmental mechanism of cephradine basing on mass spectrometry.
Key words : Cefradine stability Spectral analysis
目 录
摘 要 I
Abstract II
第一章 绪论 1
1.1课题来源 1
1.2 β-内酰胺类抗生素 1
1.2.1研究β-内酰胺类抗生素的意义 1
1.2.2 β-内酰胺类抗生素简介 1
1.2.3 β-内酰胺类抗生素化学不稳定性 3
1.2.4 β-内酰胺类抗生素化学稳定性的研究现状 4
1.3光谱分析在β-内酰胺类抗生素中的应用 6
1.3.1分析测试仪器简介 6
1.3.2光谱分析技术在β-内酰胺类化合物研究方面的应用 8
1.4 密度泛函理论现代分析测试中的应用 9
1.4.1密度泛函理论简介 9
1.4.2在β-内酰胺类抗生素研究中的应用 10
1.5头孢拉定简介 10
1.5.1头孢拉定结构式 10
1.5.2头孢拉定性质 10
1.6展望 11
第二章 实验部分 12
2.1试剂 12
2.2仪器 13
2.3 头孢拉定含量检测 13
2.3.1高效液相色谱检测 13
2.3.2质谱检测 14
2.3.3红外光谱检测 14
2.3.4紫外检测 14
2.3.5验证实验 14
第三章 光谱分析 17
3.1高效液相色谱分析方法研究 17
3.1.1系统适用性研究 17
3.1.2标准溶液的配制 19
3.1.3线性关系考察 19
3.2质谱分析 19
3.2.1正谱分析 19
3.2.2负谱分析 22
3.3红外光谱分析 24
3.4紫外光谱分析 25
第四章 影响化学稳定性因素的研究 26
4.1光照对头孢拉定化学稳定性影响 26
4.2温度对头孢拉定化学稳定性影响 26
4.3金属离子对头孢拉定化学稳定性影响 27
4.4晶型对头孢拉定化学稳定性影响 29
4.5 pH对头孢拉定稳定性的影响研究 30
结 论 31
致 谢 32
参考文献 33
Contents
Abstract I
Abstract II
ChapterⅠIntroduction 1
1.1 Source Subject 1
1.2 Antibiotics of β- lactam 1
1.2.1 Meaning of β-lactam antibiotics 1
1.2.2 Profile of β-lactam antibiotics 1
1.2.3 Chemical instability of β-lactam antibiotics 3
1.2.4 Chemical stability of the research β-lactam antibiotics 4
1.3 Application of spectrum in the β-lactam antibiotics 6
1.3.1 Introduction of analytical instruments 6
1.3.2 Application of spectrum in the β-lactam antibiotics 8
1.4 Density functional theory in the application of modern analysis 9
1.4.1Introduction of density functional theory 9
1.4.2Application of density functional theory in the β-lactam antibiotics 10
1.5 Cephradine Profile 10
1.5.1 Cephradine Structure 10
1.5.2 The Characteristics of Cephradine 10
1.6 Outlook 11
Chapter Ⅱ Experimental 12
2.1 Reagent 12
2.2 Instrument 13
2.3 Cephradine Levels Detected 13
2.3.1 Detect of High-performance liquid chromatography 13
2.3.2 Detect of Mass Spectrometry 14
2.3.3 Detect of Infrared Spectroscopy 14
2.3.4 Detect of UV-Vis 14
2.3.5 Verification Experiment 14
Chapter Ⅲ Spectrum Analysis 17
3.1 Study on Analytical Method of HPLC 17
3.1.1 Study on System Suitability 17
3.1.2 Standard Solution Preparation 19
3.1.3 Linear relationship between absorption and concentration 19
3.2 Mass Spectrometry 19
3.2.1 Analysis of Positive Spectral 19
3.2.2 Analysis of Negative Spectral 22
3.3 Analysis of Infrared Spectroscopy 24
3.4 Analysis of UV-Vis 25
Chapter Ⅳ Factors Affecting Chemical Stability 26
4.1 Effect of light on chemical stability of cephradine 26
4.2 Effect of temperature on chemical stability of cephradine 26
4.3 Effect of metalions on chemical stability of cephradine 27
4.4 Effect of crystal on chemical stability of cephradine 29
4.5 Effect of pH on chemical stability of cephradine 30
Conclusion 31
Acknow..
摘 要
头孢拉定为第一代头孢菌素类抗生素，广泛用于尿路及呼吸道感染,是目前临床应用最广泛的抗生素之一。然而，由于头孢拉定化学性质不稳定，严重地影响产品质量，甚至于影响到用药安全。
本文应用IR、UV-Vis、MS、HPLC、XRD等检测手段，采用实验、检测与理论分析相结合的方法，从光谱与结构性质、破坏性因素的影响两个层面，研究了该化合物不稳定性的原因以及破坏性因素的影响程度。
研究表明：温度和结晶水对头孢拉定影响较大，常见的金属离子中MnCl2和CoAC2会与头孢拉定发生化学反应。紫外光照和晶体形貌对于该化合物稳定性影响不大。此外，本项研究还在质谱检测的基础上，提出了全新的头孢拉定酸碱裂解机理。
关键词： 头孢拉定 、化学稳定性、光谱分析
Abstract
Cephradine is 1st generation of cephalosporins, which is used in the treatment of urinary and respiratory infection and is one of the most often used antibiotic. Cephradine is a labile compound，which influents quality of product and even the safety in utilization of drugs.
In this paper, With the combination of theoretical analysis and experiment and Applying spectrum techniques，such as IR, UV-Vis, MS, HPLC, XRD to Analyzing the instability mechanism and extent of destructive factors from spectral and structural properties and disruptive factors.
Research shows that: the temperature and the crystal water greater impact on cefradine, MnCl2 and CoAC2 have obviously complex reaction with Cephradine, UV irradiation and the crystal morphology has little effect for the stability of the compound. In addition, the study proposes a new fragmental mechanism of cephradine basing on mass spectrometry.
Key words : Cefradine stability Spectral analysis
目 录
摘 要 I
Abstract II
第一章 绪论 1
1.1课题来源 1
1.2 β-内酰胺类抗生素 1
1.2.1研究β-内酰胺类抗生素的意义 1
1.2.2 β-内酰胺类抗生素简介 1
1.2.3 β-内酰胺类抗生素化学不稳定性 3
1.2.4 β-内酰胺类抗生素化学稳定性的研究现状 4
1.3光谱分析在β-内酰胺类抗生素中的应用 6
1.3.1分析测试仪器简介 6
1.3.2光谱分析技术在β-内酰胺类化合物研究方面的应用 8
1.4 密度泛函理论现代分析测试中的应用 9
1.4.1密度泛函理论简介 9
1.4.2在β-内酰胺类抗生素研究中的应用 10
1.5头孢拉定简介 10
1.5.1头孢拉定结构式 10
1.5.2头孢拉定性质 10
1.6展望 11
第二章 实验部分 12
2.1试剂 12
2.2仪器 13
2.3 头孢拉定含量检测 13
2.3.1高效液相色谱检测 13
2.3.2质谱检测 14
2.3.3红外光谱检测 14
2.3.4紫外检测 14
2.3.5验证实验 14
第三章 光谱分析 17
3.1高效液相色谱分析方法研究 17
3.1.1系统适用性研究 17
3.1.2标准溶液的配制 19
3.1.3线性关系考察 19
3.2质谱分析 19
3.2.1正谱分析 19
3.2.2负谱分析 22
3.3红外光谱分析 24
3.4紫外光谱分析 25
第四章 影响化学稳定性因素的研究 26
4.1光照对头孢拉定化学稳定性影响 26
4.2温度对头孢拉定化学稳定性影响 26
4.3金属离子对头孢拉定化学稳定性影响 27
4.4晶型对头孢拉定化学稳定性影响 29
4.5 pH对头孢拉定稳定性的影响研究 30
结 论 31
致 谢 32
参考文献 33
Contents
Abstract I
Abstract II
ChapterⅠIntroduction 1
1.1 Source Subject 1
1.2 Antibiotics of β- lactam 1
1.2.1 Meaning of β-lactam antibiotics 1
1.2.2 Profile of β-lactam antibiotics 1
1.2.3 Chemical instability of β-lactam antibiotics 3
1.2.4 Chemical stability of the research β-lactam antibiotics 4
1.3 Application of spectrum in the β-lactam antibiotics 6
1.3.1 Introduction of analytical instruments 6
1.3.2 Application of spectrum in the β-lactam antibiotics 8
1.4 Density functional theory in the application of modern analysis 9
1.4.1Introduction of density functional theory 9
1.4.2Application of density functional theory in the β-lactam antibiotics 10
1.5 Cephradine Profile 10
1.5.1 Cephradine Structure 10
1.5.2 The Characteristics of Cephradine 10
1.6 Outlook 11
Chapter Ⅱ Experimental 12
2.1 Reagent 12
2.2 Instrument 13
2.3 Cephradine Levels Detected 13
2.3.1 Detect of High-performance liquid chromatography 13
2.3.2 Detect of Mass Spectrometry 14
2.3.3 Detect of Infrared Spectroscopy 14
2.3.4 Detect of UV-Vis 14
2.3.5 Verification Experiment 14
Chapter Ⅲ Spectrum Analysis 17
3.1 Study on Analytical Method of HPLC 17
3.1.1 Study on System Suitability 17
3.1.2 Standard Solution Preparation 19
3.1.3 Linear relationship between absorption and concentration 19
3.2 Mass Spectrometry 19
3.2.1 Analysis of Positive Spectral 19
3.2.2 Analysis of Negative Spectral 22
3.3 Analysis of Infrared Spectroscopy 24
3.4 Analysis of UV-Vis 25
Chapter Ⅳ Factors Affecting Chemical Stability 26
4.1 Effect of light on chemical stability of cephradine 26
4.2 Effect of temperature on chemical stability of cephradine 26
4.3 Effect of metalions on chemical stability of cephradine 27
4.4 Effect of crystal on chemical stability of cephradine 29
4.5 Effect of pH on chemical stability of cephradine 30
Conclusion 31
Acknow..
