鳄嘴花多萜类化合物的分离纯化及结构研究.doc

          

摘  要 鳄嘴花是一种药食两用的植物，大量的文献表明鳄嘴花具有抗癌，抗病毒，抗炎等多种药理作用，但对其化学成分的研究尚未完整，为进一步了解鳄嘴花的活性物质，本课题组对鳄嘴花的化学成分及抗癌活性展开了研究。课题组在前期工作中，对鳄嘴花进行了系统分离，得到了乙酸乙酯、正丁醇两个极性部位，并对乙酸乙酯部位经粗分后得到了几个流分。本人对这其中的两个流分进行了进一步的分离纯化，制备单体，并对单体进行结构鉴定，确定所得到化合物的结构。主要研究工作及结果如下：

（1） 通过色谱分离技术如常规的薄层层析摸索分离条件，利用正相硅胶柱，ODS柱等柱层析对对乙酸乙酯部位提取物进行分离提纯，再进一步用重结晶等方法进行纯化，制备单体化合物，分离纯化出化合物1及2。

（2） 利用HPLC为检测手段测定化合物1及2纯度，它们的HPLC结果显示纯度均达到99%以上，由此确定化合物1和2已经达到进行波谱分析的要求。

（3） 将纯度达标的单体化合物1进行波谱测定，质谱显示化合物1的分子量为427.40，结合1H-NMR、13C-NMR、DEPT、HSQC等波谱技术，通过查阅和对比文献确定化合物1为羽扇豆醇。

（4） 将纯度达标的单体化合物2进行波谱测定，通过MS确定分子为388.60，但其具体的结构仍在分析当中。

关键词 鳄嘴花；萜类；羽扇豆醇  

 

          Separation, purification and identification of Polyterpenoids of 

                 Clinacanthus nutans（burm.F）Lindauthe 

Abstract Clinacanthus nutans（burm.F）Lindau  is a kind of medicinal and edible plant, a lot of literatures show that Clinacanthus nutans（burm.F）Lindau with anti-cancer, anti-viral, anti-inflammatory and other pharmacological effects, but the study of its chemical composition is not yet complete, in order to further understand the active substance of Clinacanthus nutans（burm.F）Lindau , The research group carried out studies on the chemical constituents and antitumor activity by Clinacanthus nutans（burm.F）Lindau. The Group in the previous work, the Clinacanthus nutans（burm.F）Lindau  were isolated, the ethyl acetate, n-butanol two polar fractions, and the ethyl acetate fraction rough points obtained several stream. I did for the two fraction was purified further separation, preparation of monomer, and the structures were identified on the monomer, determine the structures of the compounds. The main research work and results are as follows:

(1) By chromatographic separation techniques such as TLC conventional fumble separation conditions, using normal phase silica gel column, ODS column chromatography of the extract of ethyl acetate fraction was isolated and purified by recrystallization, and then further purified preparation methods, compounds, separation of pure compounds 1 and 2 respectively.

(2) The compound 1 and 2 detection means purity using HPLC for the determination, The HPLC results showed that the purity reaches more than 99%, the compounds 1 and 2 have reached the requirements for spectrum analysis.

(3) The compounds 1 in the purity of standard wave spectroscopy, mass spectrometry showed that molecular was 427.40, according to 1H-NMR, 13C-NMR, DEPT, HSQC spectroscopy, by consulting literature and comparison to determine the compound 1 as lupeol.

(4) The compounds 2 in the purity of standard spectroscopic measurement, determined by MS molecule is 388.60, but the structure is still in analysis.

Keywords Clinacanthus nutans（burm.F）Lindau ; terpenoids; lupeol  

  目录

第一章 综述 1

1.1 鳄嘴花的生药学简介 1

1.2 鳄嘴花的化学成分 1

1.2.1 黄酮碳苷类 1

1.2.2 三萜皂苷类 2

1.2.3 含硫糖苷类化合物 2

1.2.4 其它化合物 3

1.3 鳄嘴花的药理作用 3

1.3.1 抗癌作用 3

1.3.2 抗病毒作用 4

1.3.3 其它药理作用 5

1.4 本文研究目的 6

第二章 鳄嘴花乙酸乙酯提取物中化学成分的研究 7

2.1 试验仪器与试剂 7

2.1.1 试验仪器 7

2.1.2试验试剂 7

2.1.3 试验材料 8

2.2 流分A纯化的试验方法与结果 8

2.2.2 化合物1的波谱鉴定 12

2.3 流分B纯化的试验方法与结果 18

2.3.1 流分B的分离纯 19

2.4 结 论 24

2.4.1 化合物1 24

2.4.2 化合物2 24

2.4.3 讨论与感悟 24

参考文献 25

致  谢 28