荧光性双层膜的制备及其在汞离子检测中的应用（毕业论文）.doc
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荧光性双层膜的制备及其在汞离子检测中的应用（毕业论文）,题目：荧光性双层膜的制备及其在汞离子检测中的应用院系：材料科学与工程学院专业：环境工程目录一前言5二实验部分62.1实验仪器与试剂62.1.1仪器62.1.2试剂62.2实验内容72.2.1自组装膜的制备72.2.1.1组装机理72.2.1.2 cys/au的制备72.2.1.3hox/cys/au自组装膜的制备72....
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题 目：荧光性双层膜的制备及其在汞离子检测中的应用
院 系：材料科学与工程学院
专 业：环境工程
目录
一 前言 5
二 实验部分 6
2.1 实验仪器与试剂 6
2.1.1 仪器 6
2.1.2 试剂 6
2.2 实验内容 7
2.2.1 自组装膜的制备 7
2.2.1.1 组装机理 7
2.2.1.2 Cys/Au的制备 7
2.2.1.3 HOX/Cys/Au自组装膜的制备 7
2.2.2 8-羟基喹啉铝（8-羟基喹啉汞）的合成 8
2.2.3 Al(OX)3/Cys/Au的制备 8
2.2.4 自组装膜荧光光谱测定 8
2.2.5 电化学实验 9
2.2.6 膜的结构表征 9
三 结果与讨论 9
3.1 8-羟基喹啉和8-羟基喹啉铝溶液溶剂、激发波长和pH的选择 9
3.1.1 溶剂的选择 9
3.1.2 8-羟基喹啉和8-羟基喹啉铝溶液的激发波长的选择 10
3.1.3 8-羟基喹啉和8-羟基喹啉铝溶液pH值的选择 10
3.2 自组装膜优化条件的选择 11
3.2.1.1 半胱胺酸在金表面上自组装的电化学表征 11
3.2.1.2 组装DL－半胱氨酸浓度的选择 12
3.2.1.3 组装DL－半胱氨酸时间的选择 12
3.2.1.4 组装DL－半胱氨酸pH值的选择 13
3.2.2 Cys/Au电极酸化时间和浓度的选择 13
3.2.3 8－羟基喹琳荧光物质及其金属螯合物的组装 13
3.2.3.1 确定荧光物质的激发波长 13
3.2.3.2 浸泡时间与荧光物质浓度的选择 14
3.2.3.3 pH值的选择 14
3.3 自组装双层膜的荧光光谱 15
3.4 自组装膜的表征 16
3.4.1 电极荧光光谱表征 16
3.4.2 膜的结构表征 16
3.5 8－羟基喹啉铝、8－羟基喹啉溶液及自组装膜稳定性能的比较 17
3.5.1 溶液荧光强度的比较 17
3.5.2 稳定性的比较 17
3.6 Hg2+的荧光传感分析 18
3.6.1 汞离子的荧光传感的分析特性 18
四 结论 20
五 参考文献 20
Abstract
Self-assembled monolayer(SAMs) can be designed according to our demand in molecular level，It has many merits which don’t exit in other methods. Now SAMs has been extensive applied in electroanalytical chemistry. But it is few reported about its application in fluorescence analysis. Fluorescence analysis is an important and effective spectrum analysis，If we can combine combine SAMs technology with fluorescence analytical method and develop another interface fluorescence analytical method，It was effective in diminishing the sample volume with a view of on-chip analysis，therefore has promising prospects in fluorescence analysis and chemical sensor.
The fluorescence intensity of 8-hydroxyquinoline is low，in order to improve its fluorescence intensity , in this paper we synthesized fluorescence reagent 8-hydroxyquinoline aluminum(Al(OX)3 , It is a very significant electroluminscent material and has attracted greated attention both from fundamental and potential application point of view .
In this experiment , The self-assembled bilayer membrane Al(OX)3/Cys/Au was fabricated via electrostatic action with DL-cysteine(Cys)monolayers on gold,surface. in order to characterized its structure , we make use of cyclic voltammetry(CV)in combination with X-ray Photoelectron spectra(XPS) .
It was found that the self-assembled bilayer membrane Al(OX)3/Cys/Au has good selectivity for Hg2+, so it can be applied to the detection of mercury ion. Moreover, we found that fluorescent signal of Al(OX)3/Cys/Au can be quenched by Hg2+.
Keyword: fluorescence self-assembled bilayer membrane
8-hydroxyquinoline mercury ion
院 系：材料科学与工程学院
专 业：环境工程
目录
一 前言 5
二 实验部分 6
2.1 实验仪器与试剂 6
2.1.1 仪器 6
2.1.2 试剂 6
2.2 实验内容 7
2.2.1 自组装膜的制备 7
2.2.1.1 组装机理 7
2.2.1.2 Cys/Au的制备 7
2.2.1.3 HOX/Cys/Au自组装膜的制备 7
2.2.2 8-羟基喹啉铝（8-羟基喹啉汞）的合成 8
2.2.3 Al(OX)3/Cys/Au的制备 8
2.2.4 自组装膜荧光光谱测定 8
2.2.5 电化学实验 9
2.2.6 膜的结构表征 9
三 结果与讨论 9
3.1 8-羟基喹啉和8-羟基喹啉铝溶液溶剂、激发波长和pH的选择 9
3.1.1 溶剂的选择 9
3.1.2 8-羟基喹啉和8-羟基喹啉铝溶液的激发波长的选择 10
3.1.3 8-羟基喹啉和8-羟基喹啉铝溶液pH值的选择 10
3.2 自组装膜优化条件的选择 11
3.2.1.1 半胱胺酸在金表面上自组装的电化学表征 11
3.2.1.2 组装DL－半胱氨酸浓度的选择 12
3.2.1.3 组装DL－半胱氨酸时间的选择 12
3.2.1.4 组装DL－半胱氨酸pH值的选择 13
3.2.2 Cys/Au电极酸化时间和浓度的选择 13
3.2.3 8－羟基喹琳荧光物质及其金属螯合物的组装 13
3.2.3.1 确定荧光物质的激发波长 13
3.2.3.2 浸泡时间与荧光物质浓度的选择 14
3.2.3.3 pH值的选择 14
3.3 自组装双层膜的荧光光谱 15
3.4 自组装膜的表征 16
3.4.1 电极荧光光谱表征 16
3.4.2 膜的结构表征 16
3.5 8－羟基喹啉铝、8－羟基喹啉溶液及自组装膜稳定性能的比较 17
3.5.1 溶液荧光强度的比较 17
3.5.2 稳定性的比较 17
3.6 Hg2+的荧光传感分析 18
3.6.1 汞离子的荧光传感的分析特性 18
四 结论 20
五 参考文献 20
Abstract
Self-assembled monolayer(SAMs) can be designed according to our demand in molecular level，It has many merits which don’t exit in other methods. Now SAMs has been extensive applied in electroanalytical chemistry. But it is few reported about its application in fluorescence analysis. Fluorescence analysis is an important and effective spectrum analysis，If we can combine combine SAMs technology with fluorescence analytical method and develop another interface fluorescence analytical method，It was effective in diminishing the sample volume with a view of on-chip analysis，therefore has promising prospects in fluorescence analysis and chemical sensor.
The fluorescence intensity of 8-hydroxyquinoline is low，in order to improve its fluorescence intensity , in this paper we synthesized fluorescence reagent 8-hydroxyquinoline aluminum(Al(OX)3 , It is a very significant electroluminscent material and has attracted greated attention both from fundamental and potential application point of view .
In this experiment , The self-assembled bilayer membrane Al(OX)3/Cys/Au was fabricated via electrostatic action with DL-cysteine(Cys)monolayers on gold,surface. in order to characterized its structure , we make use of cyclic voltammetry(CV)in combination with X-ray Photoelectron spectra(XPS) .
It was found that the self-assembled bilayer membrane Al(OX)3/Cys/Au has good selectivity for Hg2+, so it can be applied to the detection of mercury ion. Moreover, we found that fluorescent signal of Al(OX)3/Cys/Au can be quenched by Hg2+.
Keyword: fluorescence self-assembled bilayer membrane
8-hydroxyquinoline mercury ion
