毛巾上的细菌分离及鉴定.doc
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毛巾上的细菌分离及鉴定,摘要:通过本次为期四周实验,学习和掌握从周围环境中分离不同的菌种,以及对其纯化鉴定的基本操作技能,了解微生物学的基础知识,与此同时,通过对不同菌种的直观认识及其危害性的了解,提高人们对日常生活用品的防护意识,并呼吁人们科学合理的生活习惯。在本次实验中,主要通过有关微生物的基础操作性实验:细菌的大小...
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毛巾上的细菌分离及鉴定
摘要:通过本次为期四周实验,学习和掌握从周围环境中分离不同的菌种,以及对其纯化鉴定的基本操作技能,了解微生物学的基础知识,与此同时,通过对不同菌种的直观认识及其危害性的了解,提高人们对日常生活用品的防护意识,并呼吁人们科学合理的生活习惯。
在本次实验中,主要通过有关微生物的基础操作性实验:细菌的大小测定及其数目的测定、简单染色、革兰氏染色;四个基本鉴定性实验:大分子物质的水解、糖发酵试验、IMViC试验以及硫化氢试验。对所分离纯化出的不同菌种的形态和生理生化特征进行分析鉴定。经过查阅相关资料《伯杰氏细菌鉴定手册》得出最终结果:毛巾中含有革兰氏阴性的大肠杆菌,及革兰氏阳性的金黄色葡萄球菌和表皮葡萄球菌。
通过本次实验得知,在人们日常生活中,一些必需品很容易细菌的繁殖,经实验测定,平均每平方厘米的毛巾上有约120000个细菌,特别是在潮湿的毛巾上毛巾会快速繁殖,所以,毛巾很容易产生异味和发粘,正是由于毛巾上的细菌、油脂、蛋白质已经发酵、变质。因此,毛巾使用一段时间要注意消毒。
关键词:毛巾、细菌分离、鉴定、危害。
Abstract: This four-week experiment, study and master the separation from the surrounding environment in different species, and its Purification and Identification of the basic skills, learn the basics of microbiology at the same time, by different strains of the visual knowledge and understanding of the dangers to raise awareness of the daily necessities of protection consciousness, and called it a scientific and rational habits.
In this experiment, the main basis for interoperability through the microbe experiment: Determination of the number of bacteria in the determination of the size, simple stain, gram stain; four basic identification study: hydrolysis of macromolecules, sugar fermentation test , IMViC test and hydrogen sulfide. On the isolated and purified strains of different morphological and physiological and biochemical characteristics were analyzed and identified. Through access to relevant information, "Bo Jieshi bacterial identification manual" that the final results: Towels containing Gram-negative E. coli, and Gram-positive Staphylococcus aureus and Staphylococcus epidermidis.
Through this experiment that, in people's daily life, some necessities is easy reproduction of bacteria, measured by the experiment, the average per square centimeter of towels around 120 000 bacteria, especially in wet towels on the towel will quickly multiply Therefore, the towel is easy to produce odor and sticky, formally as a towel on the bacteria, fat, protein has been fermented, metamorphism. Therefore, the towel to use for some time to pay attention to disinfection.
Keywords: towel, bacterial isolation, identification, hazard.
摘要:通过本次为期四周实验,学习和掌握从周围环境中分离不同的菌种,以及对其纯化鉴定的基本操作技能,了解微生物学的基础知识,与此同时,通过对不同菌种的直观认识及其危害性的了解,提高人们对日常生活用品的防护意识,并呼吁人们科学合理的生活习惯。
在本次实验中,主要通过有关微生物的基础操作性实验:细菌的大小测定及其数目的测定、简单染色、革兰氏染色;四个基本鉴定性实验:大分子物质的水解、糖发酵试验、IMViC试验以及硫化氢试验。对所分离纯化出的不同菌种的形态和生理生化特征进行分析鉴定。经过查阅相关资料《伯杰氏细菌鉴定手册》得出最终结果:毛巾中含有革兰氏阴性的大肠杆菌,及革兰氏阳性的金黄色葡萄球菌和表皮葡萄球菌。
通过本次实验得知,在人们日常生活中,一些必需品很容易细菌的繁殖,经实验测定,平均每平方厘米的毛巾上有约120000个细菌,特别是在潮湿的毛巾上毛巾会快速繁殖,所以,毛巾很容易产生异味和发粘,正是由于毛巾上的细菌、油脂、蛋白质已经发酵、变质。因此,毛巾使用一段时间要注意消毒。
关键词:毛巾、细菌分离、鉴定、危害。
Abstract: This four-week experiment, study and master the separation from the surrounding environment in different species, and its Purification and Identification of the basic skills, learn the basics of microbiology at the same time, by different strains of the visual knowledge and understanding of the dangers to raise awareness of the daily necessities of protection consciousness, and called it a scientific and rational habits.
In this experiment, the main basis for interoperability through the microbe experiment: Determination of the number of bacteria in the determination of the size, simple stain, gram stain; four basic identification study: hydrolysis of macromolecules, sugar fermentation test , IMViC test and hydrogen sulfide. On the isolated and purified strains of different morphological and physiological and biochemical characteristics were analyzed and identified. Through access to relevant information, "Bo Jieshi bacterial identification manual" that the final results: Towels containing Gram-negative E. coli, and Gram-positive Staphylococcus aureus and Staphylococcus epidermidis.
Through this experiment that, in people's daily life, some necessities is easy reproduction of bacteria, measured by the experiment, the average per square centimeter of towels around 120 000 bacteria, especially in wet towels on the towel will quickly multiply Therefore, the towel is easy to produce odor and sticky, formally as a towel on the bacteria, fat, protein has been fermented, metamorphism. Therefore, the towel to use for some time to pay attention to disinfection.
Keywords: towel, bacterial isolation, identification, hazard.