本科微生物论文--不同酒精浓度和不同酒精倍数对灰树花265多糖提取的影响.rar

论文题目是：不同酒精浓度和不同酒精倍数对灰树花265多糖提取的影响

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目录
摘要 ………………………………………………………………………………………………3
Abstract  …………………………………………………………………………………………4
1引言 ……………………………………………………………………………………………5
2材料与方法 ……………………………………………………………………………………7
2.1 材料…………………………………………………………………………………………7
2.2试剂 …………………………………………………………………………………………7
2.3仪器设备……………………………………………………………………………………7
2.4 发酵液的处理 ……………………………………………………………………………8
2.5 酒精提取……………………………………………………………………………………8
2.6 酮-比色法 …………………………………………………………………………………8
2.6.1 原理………………………………………………………………………………………8
2.6.2 药品………………………………………………………………………………………8
2.6.3 试剂………………………………………………………………………………………8
2.6.4葡萄糖标准液……………………………………………………………………………8
2.6.5样品总糖的测定…………………………………………………………………………9
2.6.6样品还原糖的测定 ……………………………………………………………………9
2.6.7计算 ………………………………………………………………………………………9
３结果与分析 …………………………………………………………………………………10
3.1.1不同酒精倍数的醇沉样品中总糖含量测定 ……………………………………10
3.1.2不同酒精浓度的醇沉样品中总糖含量测定 ……………………………………11
3.2.1不同酒精倍数的醇沉样品中还原糖含量测定 …………………………………11 
3.2.2不同酒精浓度的醇沉样品中还原糖含量测定 …………………………………12
3.3.1不同酒精倍数的醇溶样品中总糖含量测定 ……………………………………13
3.3.2不同酒精浓度的醇溶样品中总糖含量测定 ……………………………………14
3.4.1不同酒精倍数的醇溶样品中还原糖含量测定 …………………………………14
3.4.2不同酒精浓度的醇溶样品中还原糖含量测定 …………………………………15
4分析与讨论……………………………………………………………………………………16
致谢………………………………………………………………………………………………16
参考文献 ………………………………………………………………………………………17

不同酒精浓度和不同酒精倍数对灰树花265多糖提取的影响

摘要：为了筛选出一种比较准确、方便的多糖的提取方法, 本论文采用灰树花265菌株发酵液，用四种不同酒精浓度为60%、70%、80%、90%分别进行2倍、4倍、6倍、8倍不同酒精倍数醇沉正交实验，得到16个醇沉和醇溶提取物，利用硫酸-蒽酮法测定提取物的糖含量。在不同酒精浓度比较试验中，结果显示：酒精浓度为70%时，测得糖含量较大，因此认为酒精浓度应该选取80%。在不同酒精倍数比较试验中，结果显示：酒精倍数在2- 4之间随着倍数的增大所测得糖含量逐渐增大，酒精倍数为4倍之后糖含量下降，酒精倍数为4倍时，测得糖含量最大，因此应选取4倍。
关键词：灰树花；酒精提取；蒽酮-比色法；糖含量

Different alcohol concentration of alcohol and different times of Extraction of the impact of polysaccharide
 
  
Abstract: In order to select a more accurate and convenient method of extracting the polysaccharide, the paper used Grifola frondosa 265 strain broth, with four different alcohol concentration of 60% and 70%, 80% and 90% respectively 2 Times, four times, six times, eight times the multiples of different alcohol-alcohol orthogonal experiment, received 16-and alcohol extract of Alcohol, using sulfuric acid - anthrone-Determination extract of the sugar content. Comparing the different alcohol concentration test results showed that: alcohol concentration of 70 percent, measured higher sugar content, so that the concentration of alcohol should select 80%. At different times compared alcohol test results showed that: alcohol in multiples of 2 - 4 with multiples between the increase measured by the sugar content is gradually increasing, multiples of alcohol for four times after the decline in sugar content, alcohol multiples of four times when , The largest measured sugar content and therefore should be selected four times.
Key words: Grifola frondosa; Alcohol extraction; Anthrone-- colorimetry; Sugar content