两种植物提取物对小麦幼苗两种酶活性的影响.rar

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两种植物提取物对小麦幼苗两种酶活性的影响,13页1.1万余字 [摘要] 用黄花蒿及中国粗榧浸膏作为化感物质对小麦幼苗的化感作用的测试表明,试验小麦的长势随化感物质的浓度增大而减弱。用供试样品设置梯度(中国粗榧为0.05g、0.075g和0.1g浸膏每1ml酒精,黄花蒿为0.3g、0.5g和0.8g粉末加10ml水)对小麦幼苗处理后对切过氧化物酶及淀粉酶活性进的...
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13页1.1万余字
[摘要] 用黄花蒿及中国粗榧浸膏作为化感物质对小麦幼苗的化感作用的测试表明,试验小麦的长势随化感物质的浓度增大而减弱。用供试样品设置梯度(中国粗榧为0.05g、0.075g和0.1g浸膏每1ml酒精,黄花蒿为0.3g、0.5g和0.8g粉末加10ml水)对小麦幼苗处理后对切过氧化物酶及淀粉酶活性进的测定表明,过氧化物酶(POD)活性随化感物浓度增大而增大,中国粗榧处理后过氧化物酶与对照组相比抑制率分别为-43.9%、-29.2%和-13.3%,黄花蒿抑制率分别为-61.7%、-40.0%和-25.3%;淀粉酶活性随化感物浓度变大而减小,中国粗榧处理后α淀粉酶的抑制率分别为21.0%、21.0%和37.3%,总酶活抑制率分别为8.3%、20.8%和29.2%,黄花蒿处理后α淀粉酶抑制率分别为25.7%、39.6%和51.1%,总酶活抑制率分别为12.5%、12.5%和37.5%,α淀粉酶活性的抑制率大于总酶活的抑制率。黄花蒿对小麦幼苗2种酶的抑制率比中国粗榧大。
关键词:小麦,化感,淀粉酶,过氧化物酶,黄花蒿,中国粗榧

Effect of Leaching Liquor Materials of 2 Plants in 2 Enzymes of Wheat Sprout
[Abstract] In the study on the effect on wheat about allelopathy with using Artemisia annua L. soaked out material and Cephalotaxus sinensis leaching liquor material as allelochemicals, we found the growth of wheat became weak as the allelochemicals dense more. With a grads of material for test which 0.05g,0.075g,0.1g Cephalotaxus sinensis added 1ml alcohol and 0.3g,0.5g,0.8g Artemisia annua power with 10 ml pure water, We took a test about the activity of amylase and POD on wheat sprout for the sake of making their mechanism clear. While the test , the POD became more active while the dense of allelochemicals increase, and the activity of amylase weak while the allelochemicals increase. The bate ratio of Cephalotaxus sinensis to POD activity are -43.9%、-29.2%、-13.3% in each consistency, and the Artemisia annua to it are -61.7%、-40.0%、-25.3% of each consistency. After the Cephalotaxus sinensis used in wheat ,the bate ratio to α- amylase are 21.0%、21.0%、37.3% in each consistency ,and the bate ratio to amylase are 8.3%、20.8% and 29.2%.With Artemisia annua using on wheat, the bate ratio toα- amylase are 25.7%、39.6%、51.1% in each consistency ,and the bate ratio to amylase are 12.5%、12.5%、37.5%. The efficacity of Artemisia annua to wheat in Allelopathy is stronger than what the Cephalotaxus sinensis be.
Key words: Wheat, Allelopathy , Amylase, POD, Artemisia annua L. , Cephalotaxus sinensis (Rehd. et Wile.) Li


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