小麦硫转运蛋白基因rt-pcr半定量检测体系的建立.rar

小麦硫转运蛋白基因RT-PCR半定量检测体系的建立
14页 1.3万余字，包含完整毕业设计及答辩ppt
[摘要] 应用RT-PCR技术， 以Tubulin为内参基因，小麦硫转运蛋白基因(Sul)为检测基因，优化Mg2+浓度、退火温度、循环次数、引物浓度和Taq酶浓度，获得适宜的PCR反应参数，建立一个相对稳定、准确、特异的检测Sul表达量的半定量体系，为进一步研究小麦硫转运蛋白表达调节机制奠定技术基础。

[关键词] 小麦；Tubulin；硫转运蛋白基因；RT-PCR；半定量PCR


To Establish a Method of Semiquantitative Polymerase Chain Reaction for Detecting Sul Gene Expression

Abstract To establish a method of semiquantitative polymerase chain reaction for detecting Sulphate transporter gene expression in wheat roots，we established a semiquantitative PCR by putting primer of Tubulin served as internal reference gene and primer of target gene in the same conditions and optimizing experiment parameters for PCR such as the concentration of Mg2+ and cycle times etc. This method is usable to test the expressive level of Sul gene in the wheat roots.

Key words：wheat；Sulphate transporter；RT-PCR；semiquantitative PCR







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