bt抗虫棉的研究报告.doc

Bt抗虫棉的研究报告

本文共有18页，7600余字；

摘要：

为检测Bt抗虫棉棉籽及棉籽油对哺乳动物的诱变活性，以评价转Bt基因抗虫棉的安全性，本实验以环磷酰胺作为阳性诱变剂，以普通棉的棉籽及棉籽油作为对照物，同时设阴性对照组，以成年封闭群昆明种雄性小鼠为实验材料，将棉籽粉或棉籽油掺入饲料中饲喂小鼠，检测小鼠骨髓嗜多染红细胞（Polychromatic erythrocytes , PCE）微核率（Micronucleus frequency, MN‰）和精子畸变率（Sperm malformation rate）。实验结果显示，饲喂棉籽及棉籽油饲料的实验组的微核率与阴性对照组相比，大多数P>0.05，无显著性差异，有四组P<0.05，但它们的微核率比阴性对照组小，即这四组的微核率显著小于阴性对照组；与阳性对照组相比，P<0.001，都有极显著差异。上述实验组的精子畸变率与阴性对照组相比，P>0.05，无显著性差异；与阳性对照组相比，P<0.05，有显著性差异。结果表明：在本实验条件下，Bt抗虫棉与普通棉的棉籽及棉籽油对小鼠体细胞和性细胞均未显示出诱变活性，且没有剂量效应。

关键词：Bt抗虫棉 棉籽 棉籽油 嗜多染红细胞微核率 精子畸变率

Study on Mutagenicity of Cotton Seed and Cotton Seed Oil
of Bt-transgenic Cottons

Abstract

To evaluate the safety of insect resistant cotton plants,which express a Bacillus thuringiensis protein,the mutagenicity of cotton seed and cotton seed oil on mammals was examined.The raw cotton seed and oil was mixed into mice’ diet. Using cyclophosphamide as positive control,non-modified cotton seed and cotton seed oil as controls, this study tested the micronucleus frequency in mice bone marrow polychromatic erythrocytes and the sperm malformation rate.The results showed that there’s no significant difference between most of the seed groups,oil groups and the negative control(P>0.05) or that the micronucleus frequency of the seed and oil groups is significantly lower than the negative control(P<0.05).On the other hand,there existed significant difference between the seed groups,oil groups and the positive control (micronucleus test P<0.001,sperm malformation test P<0.05).

目录


一、前言.......................................................................................1
二、材料和方法..........................................................................3
2.1 材料....................................................................................3
2.2 方法....................................................................................3
2.2.1 实验分组.....................................................................3
2.2.2 实验处理.....................................................................3
2.2.3 活体小鼠微核试验..........................................................3
2.2.4 活体小鼠精子畸形试验...................................................4
三、结果.......................................................................................4
3.1 微核试验............................................................................5
3.2 精子畸形试验....................................................................6
3.3 棉籽及棉籽油对小鼠体重增长的影响...........................8
四、讨论.....................................................................................13
五、小结.....................................................................................14
六、参考文献.............................................................................15
致谢


六 参考文献


[1] Indra K.Vasil,Milestones in crop biotechnology––Transgenic cassava and Agrobacterium–mediated transformation of maize,NATURE BIO/TECHNOLOGY, Volume 14,June 1996,702
[2] Russ Hoyle,EPA okays first pesticidal transgenic plants,BIO/TECHNOLOGY, Volume 13,May 1995,434
[3] George T.Tzotzos,GENETICALLY MODIFIED ORGANISMS(A GUIDE TO BIOSAFETY),©United Nations Industrial Development Organization (UNIDO),1995,43
[4] Juan J.Estruch,Nadine B.Carozzi,Nanili Desai,et al.Transgenic plants:An emerging approach to pest control,NATURE BIO/TECHNOLOGY,Volume 15,February 1997,137,139
[5] Margaret Mellon,Jane Rissler,Transgenic Crops:USDA Data on Small–Scale Tests Contribute Little to Commercial Risk Assessment,BIO/TECHNOLOGY, Volume13,January 1995,96