葛根总黄酮的提取分离方法研究.doc
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葛根总黄酮的提取分离方法研究,本文共7页 2996字[摘要]目的建立一套有效的提取及初步分离葛根中葛根素的方法。方法使用紫外分光光度法、薄层色谱及高效液相色谱对葛根素纯品、大豆甙元纯品和葛根的初纯化物进行比较鉴定。结果采用回流法提取、萃取法纯化是一种产量较大、纯度很高而且简单易行的方法，较适用于工业化大生产。结论回流法提...
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葛根总黄酮的提取分离方法研究
本文共7页 2996字
[摘要] 目的 建立一套有效的提取及初步分离葛根中葛根素的方法。方法 使用紫外分光光度法、薄层色谱及高效液相色谱对葛根素纯品、大豆甙元纯品和葛根的初纯化物进行比较鉴定。结果 采用回流法提取、萃取法纯化是一种产量较大、纯度很高而且简单易行的方法，较适用于工业化大生产。结论 回流法提取，萃取法纯化是一种较合适的方法。
[关键词] 葛根，黄酮，提取分离
Abstract: Objective In order to set up a set of effective Extraction and separation of Flavonoids in Radix Puerariae. Methods Reflux and Extraction were adopted. And the Flavonoids in Radix Puerariae were determined by UV spectrophotometer, Thin Layer Chromatography and High Performance Liquid Chromatography. Results Reflux is a good method of Extraction and Extraction is a effective method of Separation. Adopting them, we can get high quality extraction. They are suitable for industrial production. Conclusion It indicates that Reflux and Extraction are effective method of Extraction and Separation of Flavonoids in Radix Puerariae
Key words: Radix Pueraria; flavonoid; extraction and separation
[参考文献]
[1] 张 伟,栗晓黎,田金改. 高效液相色谱法测定鼻炎胶囊中葛根素的含量. 药物分析杂志,1994 ,14 (4) :43.
[2]张世勤,李树琦,胡长风,等. 根痛平中葛根素的含量测定. 中草药,1996 ,27 (7) :405.
[3] 谭生建,汪海东,邱 夏. RP2HPLC 测定脑得生片中葛根素含量. 中成药,1997 ,19 (5) :17.
[4] 张志荣,游学均,何 勤,等. HPLC 测定血浆中葛根素. 中国药学杂志,1997 ,32 (2) 104.
[5] 张玉洁,邵爱新. 降糖灵口服液中葛根素的含量测定. 中国实验方剂学杂志,1997 ;3 (4) :41
本文共7页 2996字
[摘要] 目的 建立一套有效的提取及初步分离葛根中葛根素的方法。方法 使用紫外分光光度法、薄层色谱及高效液相色谱对葛根素纯品、大豆甙元纯品和葛根的初纯化物进行比较鉴定。结果 采用回流法提取、萃取法纯化是一种产量较大、纯度很高而且简单易行的方法，较适用于工业化大生产。结论 回流法提取，萃取法纯化是一种较合适的方法。
[关键词] 葛根，黄酮，提取分离
Abstract: Objective In order to set up a set of effective Extraction and separation of Flavonoids in Radix Puerariae. Methods Reflux and Extraction were adopted. And the Flavonoids in Radix Puerariae were determined by UV spectrophotometer, Thin Layer Chromatography and High Performance Liquid Chromatography. Results Reflux is a good method of Extraction and Extraction is a effective method of Separation. Adopting them, we can get high quality extraction. They are suitable for industrial production. Conclusion It indicates that Reflux and Extraction are effective method of Extraction and Separation of Flavonoids in Radix Puerariae
Key words: Radix Pueraria; flavonoid; extraction and separation
[参考文献]
[1] 张 伟,栗晓黎,田金改. 高效液相色谱法测定鼻炎胶囊中葛根素的含量. 药物分析杂志,1994 ,14 (4) :43.
[2]张世勤,李树琦,胡长风,等. 根痛平中葛根素的含量测定. 中草药,1996 ,27 (7) :405.
[3] 谭生建,汪海东,邱 夏. RP2HPLC 测定脑得生片中葛根素含量. 中成药,1997 ,19 (5) :17.
[4] 张志荣,游学均,何 勤,等. HPLC 测定血浆中葛根素. 中国药学杂志,1997 ,32 (2) 104.
[5] 张玉洁,邵爱新. 降糖灵口服液中葛根素的含量测定. 中国实验方剂学杂志,1997 ;3 (4) :41
