蚕沙卟啉铜钠盐提取实验研究.rar
蚕沙卟啉铜钠盐提取实验研究,摘 要：植桑养蚕是我国一些地区的传统产业，在养蚕过程年产近百万吨的蚕沙。为了合理利用蚕沙资源，本课题主要研究了制备叶绿素铜钠盐的新工艺，并优化了工艺条件，以指导工业生产。首先对原料蚕沙进行叶绿素提取率的测定，并将微波提取法与普通溶剂提取法进行比较。结论证明，微波提取法的提取率要比普通溶剂提取法的提取率高出一倍。其次考察...
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摘 要：植桑养蚕是我国一些地区的传统产业，在养蚕过程年产近百万吨的蚕沙。为了合理利用蚕沙资源，本课题主要研究了制备叶绿素铜钠盐的新工艺，并优化了工艺条件，以指导工业生产。首先对原料蚕沙进行叶绿素提取率的测定，并将微波提取法与普通溶剂提取法进行比较。结论证明，微波提取法的提取率要比普通溶剂提取法的提取率高出一倍。其次考察了预处理过程中微波辐射时间、溶剂用量、溶剂与物料比例等因素的影响，确定初步的蚕沙萃取工艺条件，并优化相关的工艺条件。在试验范围内所得的数据表明:在微波辐射时间90s、破壁剂浓度40%、破壁助剂用量1：2(V[ml]:W[g]) 、提取温度60min的条件下，提取效果最佳。最后，研究了所提取的叶绿素溶液皂化条件，并摸索了叶绿素铜钠盐的制取条件。发现分离操作中，温度40℃、皂化60min效果明显，在酸性pH值为2.4的条件下铜代的60min效果较好，得到的产品品质也好。
关键词:蚕沙；微波辅助萃取;金属卟啉
Research on porphyrin-Copper-Sodium separate from silkworm
Student’ s name: DU qiangwei Advisor: WANG Shicai
（School of Biological and Chemical Engineering Zhejiang University of Science and Technology）
Abstract: For rationally utilizing silkworm faeces，it was primarily researched in this article on the new processing of preparing sodium copper chlorophyllin from silk worm and the optimal processing faeces to coaching industry production. Firstly,some work has been done on the chlorophyll extraction rate from silkworm with two different method.The conclusion proves that the extraction rate of microwave assistant extacrtion is two times than the conventional one.In this study，the influence factors were studied including irradiated time，volume of solvent，co-agent proportion etc. during the microwave pretreatment procedure.And by these investigations，tussah extracted condition was determined and optimiezd. The results indicated best that under the following conditions:irradiaetd time 90s，the concentration of cell wall crack agent 40%，the ratio of material and cell wall crack agent is 1：2(V[ml]:W[g])，extracted temperature 60℃.Lastly，the operating conditions were found for chlorophyll solvent separation and chlorophyllin-copper-sodium preparation. The excellent Saponification effect can be obtained under the following parameters:tempeartuer 40 saponated time 60min; Under the acidity condition，the yieldis higher after 60 min copper exchanged reaction.
Keywords：silkworm；microwave assistant extraction; metalloporphyrin
目 录
中文摘要 I
英文摘要 II
目录 III
1 绪论 1
1.1 蚕沙简介 1
1.1.1蚕沙的主要成分 1
1.1.2蚕沙的应用概况 1
1.2卟啉及金属卟啉简介 2
1.2.1结构及性质分析 2
1.2.2 卟啉及金属卟啉化合物的合成 3
1.2.3应用概况 5
1.3课题研究意义 7
2 试验部分 8
2.1试验所需药品和仪器列表 8
2.1.1主要化学药品 8
2.1.2主要试验仪器 8
2.2实验原理及工艺流程图 8
2.2.1实验原理 8
2.2.2工艺流程图 9
2.3蚕沙中叶绿素提取工艺条件 10
2.3.1单因素试验 10
2.3.2 正交试验 11
2.4叶绿素制取卟啉铜钠盐的实验 12
2.4.1皂化试验 12
2.4.2 铜代试验 12
2.5 分析检测方法 13
2.5.1分光光度法测量叶绿素含量 13
2.5.2卟啉铜钠盐的测定 13
3 结果与讨论 14
3.1蚕沙中叶绿素含量测定结果 14
3.2微波提取法与溶剂提取法的对比试验 14
3.3蚕沙中叶绿素提取试验 15
3.3.1单因素试验结果 15
3.3.2正交试验 19
3.4叶绿素制取卟啉铜钠盐的试验 20
3.4.1皂化试验结果分析 20
3.4.2铜代试验结果分析 22
3.4.3卟啉铜钠盐的检测结果 23
4 总结与展望 26
致 谢 27
参考文献 28
关键词:蚕沙；微波辅助萃取;金属卟啉
Research on porphyrin-Copper-Sodium separate from silkworm
Student’ s name: DU qiangwei Advisor: WANG Shicai
（School of Biological and Chemical Engineering Zhejiang University of Science and Technology）
Abstract: For rationally utilizing silkworm faeces，it was primarily researched in this article on the new processing of preparing sodium copper chlorophyllin from silk worm and the optimal processing faeces to coaching industry production. Firstly,some work has been done on the chlorophyll extraction rate from silkworm with two different method.The conclusion proves that the extraction rate of microwave assistant extacrtion is two times than the conventional one.In this study，the influence factors were studied including irradiated time，volume of solvent，co-agent proportion etc. during the microwave pretreatment procedure.And by these investigations，tussah extracted condition was determined and optimiezd. The results indicated best that under the following conditions:irradiaetd time 90s，the concentration of cell wall crack agent 40%，the ratio of material and cell wall crack agent is 1：2(V[ml]:W[g])，extracted temperature 60℃.Lastly，the operating conditions were found for chlorophyll solvent separation and chlorophyllin-copper-sodium preparation. The excellent Saponification effect can be obtained under the following parameters:tempeartuer 40 saponated time 60min; Under the acidity condition，the yieldis higher after 60 min copper exchanged reaction.
Keywords：silkworm；microwave assistant extraction; metalloporphyrin
目 录
中文摘要 I
英文摘要 II
目录 III
1 绪论 1
1.1 蚕沙简介 1
1.1.1蚕沙的主要成分 1
1.1.2蚕沙的应用概况 1
1.2卟啉及金属卟啉简介 2
1.2.1结构及性质分析 2
1.2.2 卟啉及金属卟啉化合物的合成 3
1.2.3应用概况 5
1.3课题研究意义 7
2 试验部分 8
2.1试验所需药品和仪器列表 8
2.1.1主要化学药品 8
2.1.2主要试验仪器 8
2.2实验原理及工艺流程图 8
2.2.1实验原理 8
2.2.2工艺流程图 9
2.3蚕沙中叶绿素提取工艺条件 10
2.3.1单因素试验 10
2.3.2 正交试验 11
2.4叶绿素制取卟啉铜钠盐的实验 12
2.4.1皂化试验 12
2.4.2 铜代试验 12
2.5 分析检测方法 13
2.5.1分光光度法测量叶绿素含量 13
2.5.2卟啉铜钠盐的测定 13
3 结果与讨论 14
3.1蚕沙中叶绿素含量测定结果 14
3.2微波提取法与溶剂提取法的对比试验 14
3.3蚕沙中叶绿素提取试验 15
3.3.1单因素试验结果 15
3.3.2正交试验 19
3.4叶绿素制取卟啉铜钠盐的试验 20
3.4.1皂化试验结果分析 20
3.4.2铜代试验结果分析 22
3.4.3卟啉铜钠盐的检测结果 23
4 总结与展望 26
致 谢 27
参考文献 28
