中药成分对lps诱导流产大鼠作用机理的研究------外文翻译.doc
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中药成分对lps诱导流产大鼠作用机理的研究------外文翻译,effect and mechanism of some valid components of chinese herbs in the lps-induced abortion ratsgraduate student:wei yanmingsupervisor:prof. zhao xingxupregnancy...
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Effect and Mechanism of some Valid Components of Chinese Herbs in the LPS-Induced Abortion Rats
Graduate student:Wei Yanming
Supervisor:Prof. Zhao Xingxu
Pregnancy is a complex process regulated precisely at multilevels by numerous factors such as hormones, cytolcines and other molecules, however, the functions of most these factors during pregnancy has not been fully elucidated. The etiological causes of recurrent reproductive failure are diverse, many of them are not evident in routine clinical practice and some are still unknown to sophisticated researchers in the field of reproduction medicine. Generally the causes of recurrent pregnancy loss are classified as genetic, endocrinologic, anatomic, immunologic, microbiologic, and environmental. There is much evidence that cytokines play a vital role in the establishment and maintenance of pregnancy by modulating immune and endocrine systems. Evidence from many studies on marine and human pregnancy points to a strong association between maternal Th2-type immunity and successful pregnancy on the one hand and between Thl-type immune reactivity and pregnancy loss on the other. Th2-type immunity cells may play protective roles during pregnancy. Progesterone and estrogen are central to the maintenance of pregnancy, and is thus the ideal target for fertility regulation. Progesterone and estrogen have been known to exert extensive effects via progesterone receptor (PR) and estrogen receptor (ER) on diverse physiological and developmental functions of the pregnancy,meanwhile,the receptor expression patterns change dramatically during pregnancy process.
早期胚胎死亡或胚胎丢失，又称隐性流产，严重影响母畜繁殖力与生产力。在人医临床上，自然流产的发生率占全部妊娠15%左右，多数为早期流产。妊娠时母体能够耐受同时带有父方遗传基因与母方遗传基因的半同种异体胎儿，是极其复杂而又受到精细调控的免疫学现象。许多证据表明Thl和Th2细胞因子在妊娠过程起着调节免疫和内分泌的关键作用。雌激素和孕激素在母畜怀孕过程以至整个生殖活动和其他生理活动过程中均具有重要作用，雌激素受体和孕激素受体则是实现这些功能所必需的基本元件。
为了研究中药或中药成分对实验性流产大鼠保胎的作用及其机理，本实验采用怀孕7天大鼠尾静脉注射细菌脂多糖，建立SD大鼠流产模型，再随机分为正常对照组、流产模型组、黄芪多糖组、淫羊藿组和白术黄芩组。分别于怀孕4~9天口服黄芪多糖、淫羊藿提取物和白术黄芩2ml/日，对照组与模型组分别于4~9天口服生理盐水2m1/日。于实验第10天麻醉处死大鼠，计数妊娠成功率和流产率，并以双抗体夹心ELISA法检测血浆和子宫组织匀浆中Thl型细胞因子IL-2, TNF-α和IFN-y,Th2型细胞因子IL-4, IL-10及子宫组织匀浆中ER, PR的含量变化，用RT-PCR法检测子宫组织匀浆中ER mRNA和PR mRNA的表达变化，同时，用光镜观察子宫组织结构的病理学变化，研究黄芪多糖、淫羊藿提取物和白术黄芩水煎液干预对妊娠结局和以上指标的影响。本研究表明:
1 .LPS诱导大鼠流产模型流产率高达72.73%，对照组为8.33%，两者差异极显著(P<0.01)。
2.流产模型组大鼠血浆和子宫组织匀浆Th1型细胞因子IL-2、TNF-α和IFN-y含量显
Graduate student:Wei Yanming
Supervisor:Prof. Zhao Xingxu
Pregnancy is a complex process regulated precisely at multilevels by numerous factors such as hormones, cytolcines and other molecules, however, the functions of most these factors during pregnancy has not been fully elucidated. The etiological causes of recurrent reproductive failure are diverse, many of them are not evident in routine clinical practice and some are still unknown to sophisticated researchers in the field of reproduction medicine. Generally the causes of recurrent pregnancy loss are classified as genetic, endocrinologic, anatomic, immunologic, microbiologic, and environmental. There is much evidence that cytokines play a vital role in the establishment and maintenance of pregnancy by modulating immune and endocrine systems. Evidence from many studies on marine and human pregnancy points to a strong association between maternal Th2-type immunity and successful pregnancy on the one hand and between Thl-type immune reactivity and pregnancy loss on the other. Th2-type immunity cells may play protective roles during pregnancy. Progesterone and estrogen are central to the maintenance of pregnancy, and is thus the ideal target for fertility regulation. Progesterone and estrogen have been known to exert extensive effects via progesterone receptor (PR) and estrogen receptor (ER) on diverse physiological and developmental functions of the pregnancy,meanwhile,the receptor expression patterns change dramatically during pregnancy process.
早期胚胎死亡或胚胎丢失，又称隐性流产，严重影响母畜繁殖力与生产力。在人医临床上，自然流产的发生率占全部妊娠15%左右，多数为早期流产。妊娠时母体能够耐受同时带有父方遗传基因与母方遗传基因的半同种异体胎儿，是极其复杂而又受到精细调控的免疫学现象。许多证据表明Thl和Th2细胞因子在妊娠过程起着调节免疫和内分泌的关键作用。雌激素和孕激素在母畜怀孕过程以至整个生殖活动和其他生理活动过程中均具有重要作用，雌激素受体和孕激素受体则是实现这些功能所必需的基本元件。
为了研究中药或中药成分对实验性流产大鼠保胎的作用及其机理，本实验采用怀孕7天大鼠尾静脉注射细菌脂多糖，建立SD大鼠流产模型，再随机分为正常对照组、流产模型组、黄芪多糖组、淫羊藿组和白术黄芩组。分别于怀孕4~9天口服黄芪多糖、淫羊藿提取物和白术黄芩2ml/日，对照组与模型组分别于4~9天口服生理盐水2m1/日。于实验第10天麻醉处死大鼠，计数妊娠成功率和流产率，并以双抗体夹心ELISA法检测血浆和子宫组织匀浆中Thl型细胞因子IL-2, TNF-α和IFN-y,Th2型细胞因子IL-4, IL-10及子宫组织匀浆中ER, PR的含量变化，用RT-PCR法检测子宫组织匀浆中ER mRNA和PR mRNA的表达变化，同时，用光镜观察子宫组织结构的病理学变化，研究黄芪多糖、淫羊藿提取物和白术黄芩水煎液干预对妊娠结局和以上指标的影响。本研究表明:
1 .LPS诱导大鼠流产模型流产率高达72.73%，对照组为8.33%，两者差异极显著(P<0.01)。
2.流产模型组大鼠血浆和子宫组织匀浆Th1型细胞因子IL-2、TNF-α和IFN-y含量显
