运用16srrna基因序列测定技术对羊胚胎胃肠 毕业论文.doc
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运用16srrna基因序列测定技术对羊胚胎胃肠 毕业论文,言···························································································································11.1 研究的目的与意义·······················...
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言···························································································································1
1.1 研究的目的与意义·······························································································1
1.2宿主与胃肠道微生物的关系················································································1
1.3 16SrRNA基因检测技术的产生···········································································2
1.4 16SrRNA基因检测技术的应用···········································································2
2 材料与方法··············································································································3
2.1 材料·······················································································································3
2.1.1 实验动物············································································································3
2.1.2 生化及分子生物学试剂····················································································3
2.1.3 肠道基因组DNA提取所需溶液·····································································3
2.1.4 实验主要器材····································································································4
2.2 方法·······················································································································4
2.2.1羊胚胎胃肠道细菌分离纯化·············································································4
2.2.2 分离细菌的菌落及形态特征············································································4
2.2.3 16SrRNA基因同源性分析鉴定分离细菌·························································5
2.2.3.1 分离细菌基因组DNA的提取······································································5
2.2.3.2 分离细菌16SrRNA基因的PCR扩增·························································5
2.2.3.3 PCR产物的回收与纯化·················································································5
2.2.3.4 16SrDNA文库的构建与筛选········································································6
2.2.3.5 重组阳性克隆的鉴定及测序········································································7
2.2.3.6 重组阳性克隆的酶切及RCR鉴定······························································8
2.2.3.7 16SrRNA基因序列测定及同源性分析························································8
3 结果与讨论·············································································································9
3.1 结果······················································································································9
3.1.1 分离细菌DNA提取·············································································..
1.1 研究的目的与意义·······························································································1
1.2宿主与胃肠道微生物的关系················································································1
1.3 16SrRNA基因检测技术的产生···········································································2
1.4 16SrRNA基因检测技术的应用···········································································2
2 材料与方法··············································································································3
2.1 材料·······················································································································3
2.1.1 实验动物············································································································3
2.1.2 生化及分子生物学试剂····················································································3
2.1.3 肠道基因组DNA提取所需溶液·····································································3
2.1.4 实验主要器材····································································································4
2.2 方法·······················································································································4
2.2.1羊胚胎胃肠道细菌分离纯化·············································································4
2.2.2 分离细菌的菌落及形态特征············································································4
2.2.3 16SrRNA基因同源性分析鉴定分离细菌·························································5
2.2.3.1 分离细菌基因组DNA的提取······································································5
2.2.3.2 分离细菌16SrRNA基因的PCR扩增·························································5
2.2.3.3 PCR产物的回收与纯化·················································································5
2.2.3.4 16SrDNA文库的构建与筛选········································································6
2.2.3.5 重组阳性克隆的鉴定及测序········································································7
2.2.3.6 重组阳性克隆的酶切及RCR鉴定······························································8
2.2.3.7 16SrRNA基因序列测定及同源性分析························································8
3 结果与讨论·············································································································9
3.1 结果······················································································································9
3.1.1 分离细菌DNA提取·············································································..
