食品中含有转基因成分的抽样调查.doc
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食品中含有转基因成分的抽样调查,页数 14字数 9179 [摘要]目的：调查浙江省市售食品中含有转基因食品的比率. 方法：采用sds沉淀法,提取食品中dna,经pcr扩增, 用转基因植物通常含有的35s启动子、nos终止子作为转基因成分的指示标记。结果：sds沉淀法得到的dna提取物od260／od280比值在0.4-1...
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食品中含有转基因成分的抽样调查
页数 14 字数 9179
[摘要] 目的： 调查浙江省市售食品中含有转基因食品的比率. 方法：采用SDS沉淀法,提取食品中DNA,经PCR扩增, 用转基因植物通常含有的35S启动子、NOS终止子作为转基因成分的指示标记。结果：SDS沉淀法得到的DNA提取物OD260／OD280比值在0.4-1.9, 2份转基因样品和一些食品出现阳性条带，大部分采样食品未产生阳性条带。结论：2005年浙江省市场上有转基因食品的上市,阳性率达21.3%。
[关键词] 转基因食品,SDS,PCR,35S启动子,NOS终止子
[Abstract] Objective：Investigate what percentage of Genetically Modified in food in ZheJiang province and city market.Methods：SDS methed is used for assaging transgenic component 35S promoter deriveed form Canliower Mosaic Virus and NOS transgenic from Agrobatcilum tumefacients in foods,agarosegel electrophiresis apart from production. Results：The OD26o／OD280 of DNA extraction by SDS methods Was all in the range of 1.4-1.9 ,two samples(the DNA extraction from GM foods by SDS method)and some foods showed s positive results,while others showed negative result.Conclusion：Genetically Modified Foods are sold in ZheJiang province and city market in 2005, masculine rate is 21.3%.
[Key Words] Genetically Modified Foods；SDS;PCR;CaMV35S;NOS.
[参考文献]
[1]陈 颖 ，陈 哲 ，徐宝梁 ，葛毅强 玉米加工食品中转基因成分的PCR检测
粮食与饲料工业／2OO3年靠2期 44-46
[2] Gert van Duijn，Ria van Biert，Henrieette Bleeker-Marcelis，et a1．Detection methods for genetically modified crops [J]．Food Control，1999，10：375— 378．
[3] Rogan G J，Dudin Y A，Lee T C eta1．Immunodiagnostic methods for detection of 5-enolpyruvylshikimate-3-phosphate synthase in Roundup Ready.soybeans[J]．Food Control，1999．10：407— 4l4．
[4] Joint FAO／WHO food standards programme codex committee on methods of analysis and sampling twenty-fourth session 18-22 November 2002，Budapest，Hungary[EB／OL]．http：／／flairflow4．vscht．cz／al03．23e．pdf．2003—08—01．
页数 14 字数 9179
[摘要] 目的： 调查浙江省市售食品中含有转基因食品的比率. 方法：采用SDS沉淀法,提取食品中DNA,经PCR扩增, 用转基因植物通常含有的35S启动子、NOS终止子作为转基因成分的指示标记。结果：SDS沉淀法得到的DNA提取物OD260／OD280比值在0.4-1.9, 2份转基因样品和一些食品出现阳性条带，大部分采样食品未产生阳性条带。结论：2005年浙江省市场上有转基因食品的上市,阳性率达21.3%。
[关键词] 转基因食品,SDS,PCR,35S启动子,NOS终止子
[Abstract] Objective：Investigate what percentage of Genetically Modified in food in ZheJiang province and city market.Methods：SDS methed is used for assaging transgenic component 35S promoter deriveed form Canliower Mosaic Virus and NOS transgenic from Agrobatcilum tumefacients in foods,agarosegel electrophiresis apart from production. Results：The OD26o／OD280 of DNA extraction by SDS methods Was all in the range of 1.4-1.9 ,two samples(the DNA extraction from GM foods by SDS method)and some foods showed s positive results,while others showed negative result.Conclusion：Genetically Modified Foods are sold in ZheJiang province and city market in 2005, masculine rate is 21.3%.
[Key Words] Genetically Modified Foods；SDS;PCR;CaMV35S;NOS.
[参考文献]
[1]陈 颖 ，陈 哲 ，徐宝梁 ，葛毅强 玉米加工食品中转基因成分的PCR检测
粮食与饲料工业／2OO3年靠2期 44-46
[2] Gert van Duijn，Ria van Biert，Henrieette Bleeker-Marcelis，et a1．Detection methods for genetically modified crops [J]．Food Control，1999，10：375— 378．
[3] Rogan G J，Dudin Y A，Lee T C eta1．Immunodiagnostic methods for detection of 5-enolpyruvylshikimate-3-phosphate synthase in Roundup Ready.soybeans[J]．Food Control，1999．10：407— 4l4．
[4] Joint FAO／WHO food standards programme codex committee on methods of analysis and sampling twenty-fourth session 18-22 November 2002，Budapest，Hungary[EB／OL]．http：／／flairflow4．vscht．cz／al03．23e．pdf．2003—08—01．
