豆渣异黄酮提取工艺的响应曲面优化法.doc

摘  要
　　　大豆异黄酮是从大豆中提取出来的一类微量活性物质，具有多种重要的生理功能。本论文以豆渣为实验材料，研究了大豆异黄酮的提取工艺。
　　　有机溶剂萃取法或浸泡提取法是提取大豆异黄酮的一种常用的提取方法。本实验以乙醇为溶剂，大豆豆渣为原料，研究了大豆异黄酮的提取工艺，选取了浓度、提取时间、液料比、温度四个变量对大豆异黄酮提取工艺的影响，并进行了响应曲面法优化分析，建立了相关数学模型。研究结果表明，乙醇浓度、温度、提取时间、液料比对豆渣中大豆异黄酮的提取均产生不同程度的影响。从实验结果得知，乙醇浓度对豆渣异黄酮提取量的影响最大，提取时间对大豆异黄酮提取量的影响最小。通过响应曲面法得出大豆异黄酮提取的最佳工艺条件，乙醇浓度70%、温度60℃、提取时间5h、液料比16mL/g。此时提取量可达7.72mg/10g，与实验值7.68mg/10g基本吻合。因此，该实验方案具有一定的可行性，该模型具有一定的理论价值，可为相关工业生产提供一定的理论依据。
　　　
关键词：大豆异黄酮  豆渣  提取率  响应曲面法优化
　　　

　　Optimization of isoflavones extraction from soybean dregs using response surface methodology
ABSTRACT
　　　　
　　　Soybean isoflavones from soybean extracted trace active substances,has many important physiological functions.Bean dregs as experimental material,this paper studied the soybean isoflavone extraction technology.
　　　Organic solvent extraction or soaking extraction is a king of commonly used to extract soybean isoflavone extraction method.This experiment with ethanol as solvent,soy bean dregs as raw material,soybean isoflavone extraction technology was studied and selected the concentration,extraction time and solid-liquid ratio,temperature four variables impact on soybean isoflavone extraction technology,and has carried on the response surface method optimization analysis,the relevant mathematical model is established.The research results show that ethanol concentration,temperature,extraction time and solid-liquid ratio on bean dregs in the extraction of soybean isoflavones are varying degress of impact.From the experiment results indicate that the ethanol concentration affects the quantity of bean dregs isoflavone extraction,the largest amount of time for extracting soybean isoflavines minimal impact. By response surface method for optimum process condition of soybean isoflavones extract,ethanod concentration 70%,5h temperature 60℃,  extraction time, liquid than 16 ml/g. Extract the quantity at this time can be up to 7.72 mg/g, with the experimental value of 7.68 mg / 10 g. This experiment scheme has certain feasibility, therefore, has certain theory value, the model can provide certain theoretical basis for related industrial production.
　　　                 
　　　Keywords: soybean isoflavone; bean dregs; extraction yield; response surface methodoloy 

目  录
摘  要 I
Abstract II
目  录 III
1.绪  论 1
1.1大豆异黄酮研究概况 1
1.1.1大豆异黄酮的简要概述 1
1.1.2大豆异黄酮的成分及分布 1
1.1.3豆渣异黄酮测定方法 3
1.1.4豆渣异黄酮提取工艺的研究状况 4
1.2选题意义及研究内容 5
2.实验部分 6
2.1实验仪器、材料和试剂 6
2.1.1实验仪器 6
2.1.2实验材料 6
2.1.3 实验试剂 6
2.2实验内容 7
2.2.1 豆渣异黄酮提取工艺流程 7
2.3实验方法 7
2.3.1原料预处理 7
2.3.2豆渣脱脂 7
2.3.3干燥 8
2.3.3加热浸提 8
2.3.4料液过滤 8
2.3.5异黄酮的含量测定方法 8
3．结果与分析 10
3.1单因素试验法 10
3.1.1乙醇浓度对大豆异黄酮提取的影响 10
3.1.2温度对大豆异黄酮提取量的影响 11
3.1.3提取时间对大豆异黄酮提取量的影响 11
3.1.4料液比对大豆异黄酮提取量的影响 12
3.2豆渣异黄酮的响应曲面优化法 13
3.2.1中心组合(CCD)试验设计 13
3.2.2模型的建立及其显著性检验 15
3.2.3豆渣异黄酮提取的响应曲面分析与优化 16
结  论 21
参考文献 22
致  谢 23