植物染色体制片技术的比较研究.doc
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植物染色体制片技术的比较研究,页数 3 字数 2903 摘要：本文以韭兰为材料，对植物染色体常规压片，去壁低渗法制片技术、染色效果进行了比较。实验表明：去壁低渗法获得细胞和染色体都较分散，giemsa染色使得背景与材料的对比度较大，便与观察。不足之处在于去壁低渗过程中，时间特别不易掌握，耗材多，且成功率较低。染色体容易丢...
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植物染色体制片技术的比较研究
页数 3 字数 2903
摘要：本文以韭兰为材料，对植物染色体常规压片，去壁低渗法制片技术、染色效果进行了比较。实验表明：去壁低渗法获得细胞和染色体都较分散，Giemsa染色使得背景与材料的对比度较大，便与观察。不足之处在于去壁低渗过程中，时间特别不易掌握，耗材多，且成功率较低。染色体容易丢失、形态也不太理想。常规压片耗材少，成功率较高，但不易做分带制片。
关键词 植物染色体,制片技术,去壁低渗,常规压片
This paper with Jiu orchid is material, for plant chromosome conventional sheeting, go to wall low seep legal system flat technology and dyeing effect compare . test show : go to wall low seep law get cell and chromosome scatter comparatively, Giemsa dyeing make background be greater, convenient with the contrast ratio of material with observe . is below is in in go to wall low seep process in, time do not be easy especially grasp , consumed material much, succeed just lead lower. chromosome lose easily , form also not quite ideal. conventional sheeting consumed material lose , successful rate higher, but do not be easy do minute take production
Key words:plant ;technique of making chromosome; way of ridding cell wall and putting low osmosis
[6] 王新民 再谈植物染色体标本制备的去壁、低渗法[J]
页数 3 字数 2903
摘要：本文以韭兰为材料，对植物染色体常规压片，去壁低渗法制片技术、染色效果进行了比较。实验表明：去壁低渗法获得细胞和染色体都较分散，Giemsa染色使得背景与材料的对比度较大，便与观察。不足之处在于去壁低渗过程中，时间特别不易掌握，耗材多，且成功率较低。染色体容易丢失、形态也不太理想。常规压片耗材少，成功率较高，但不易做分带制片。
关键词 植物染色体,制片技术,去壁低渗,常规压片
This paper with Jiu orchid is material, for plant chromosome conventional sheeting, go to wall low seep legal system flat technology and dyeing effect compare . test show : go to wall low seep law get cell and chromosome scatter comparatively, Giemsa dyeing make background be greater, convenient with the contrast ratio of material with observe . is below is in in go to wall low seep process in, time do not be easy especially grasp , consumed material much, succeed just lead lower. chromosome lose easily , form also not quite ideal. conventional sheeting consumed material lose , successful rate higher, but do not be easy do minute take production
Key words:plant ;technique of making chromosome; way of ridding cell wall and putting low osmosis
[6] 王新民 再谈植物染色体标本制备的去壁、低渗法[J]
