千层塔中石杉碱甲的分离、纯化研究报告.doc
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千层塔中石杉碱甲的分离、纯化研究报告,本文共计10页，6000字；摘要本文研究了从千层塔中分离、纯化石杉碱甲的方法。发现采用大孔吸附树脂法、液-液萃取及反萃取方法可较好的分离、纯化石杉碱甲。研究结果表明：将石杉碱甲粗提液ph调至7.5后上柱，采用ph为5.0、浓度50%的乙醇进行洗脱，柱分离效果最好；柱分离产品的得率和纯...
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千层塔中石杉碱甲的分离、纯化研究报告
本文共计10页，6000字；
摘 要
本文研究了从千层塔中分离、纯化石杉碱甲的方法。发现采用大孔吸附树脂法、液-液萃取及反萃取方法可较好的分离、纯化石杉碱甲。研究结果表明：将石杉碱甲粗提液pH调至7.5后上柱，
采用pH为5.0、浓度50%的乙醇进行洗脱，柱分离效果最好；柱分离产品的得率和纯度分别为0.6113%和16.77%。然后将分离液在pH3.0及pH9.0时分别用氯仿进行萃取，可得到石杉碱甲含量较高的萃取物；萃取后产品的得率和纯度分别为0.1495％和52.23％。
关键词
千层塔,石杉碱甲,分离,纯化
Abstract
The method of isolation and purification of Huperzine A from the Huperzia serrata was studied in this paper. It was found that the macroporous absorption resins, the liquid-liquid extraction and the reverse extraction could isolate and purify Huperzine A effectively. The results showed: the best column isolation condition of Huperzine A is that the pH value of the roughly extracted from Huperzia serrata was adjusted to 7.5 and then eluted by 50% ethanol resolution at pH5.0; The yield rate and purity of the isolated product were 0.6113% and 16.77% respectively. Then the pH value of the isolated of Huperzia serrata was adjusted to 3.0 and 9.0 and extracted by chloroform respectively; the content of Hup A of the product was higher. The yield rate and purity of the purified product were 0.1495% and 52.23% respectively.
目录：
前言
１．材料与方法
２．结果与分析
2.1 石杉碱甲柱分离条件的研究
2.2 萃取条件的研究
3．结论
致 谢
部分参考文献
[1] 鲁润龙，周忠泽，鲍时来等．药用植物蛇足石杉的生物学特性[J]．中国科技大学报．1999，29(1)：
118-121
[2] 钱伯初，周芝芳．改善学习记忆新药石杉碱甲研究进展[J]．浙江省医学科学院学报．1999，38：37-40
[3] 王月娥，岳冬贤，唐希灿. 石杉碱甲的抗胆碱酯酶作用. 中国药理学报.1986，7(2)：110-113
[4] Sahelian R. Huperzine A [J]. Better nutrition.1999，61(5)：26
本文共计10页，6000字；
摘 要
本文研究了从千层塔中分离、纯化石杉碱甲的方法。发现采用大孔吸附树脂法、液-液萃取及反萃取方法可较好的分离、纯化石杉碱甲。研究结果表明：将石杉碱甲粗提液pH调至7.5后上柱，
采用pH为5.0、浓度50%的乙醇进行洗脱，柱分离效果最好；柱分离产品的得率和纯度分别为0.6113%和16.77%。然后将分离液在pH3.0及pH9.0时分别用氯仿进行萃取，可得到石杉碱甲含量较高的萃取物；萃取后产品的得率和纯度分别为0.1495％和52.23％。
关键词
千层塔,石杉碱甲,分离,纯化
Abstract
The method of isolation and purification of Huperzine A from the Huperzia serrata was studied in this paper. It was found that the macroporous absorption resins, the liquid-liquid extraction and the reverse extraction could isolate and purify Huperzine A effectively. The results showed: the best column isolation condition of Huperzine A is that the pH value of the roughly extracted from Huperzia serrata was adjusted to 7.5 and then eluted by 50% ethanol resolution at pH5.0; The yield rate and purity of the isolated product were 0.6113% and 16.77% respectively. Then the pH value of the isolated of Huperzia serrata was adjusted to 3.0 and 9.0 and extracted by chloroform respectively; the content of Hup A of the product was higher. The yield rate and purity of the purified product were 0.1495% and 52.23% respectively.
目录：
前言
１．材料与方法
２．结果与分析
2.1 石杉碱甲柱分离条件的研究
2.2 萃取条件的研究
3．结论
致 谢
部分参考文献
[1] 鲁润龙，周忠泽，鲍时来等．药用植物蛇足石杉的生物学特性[J]．中国科技大学报．1999，29(1)：
118-121
[2] 钱伯初，周芝芳．改善学习记忆新药石杉碱甲研究进展[J]．浙江省医学科学院学报．1999，38：37-40
[3] 王月娥，岳冬贤，唐希灿. 石杉碱甲的抗胆碱酯酶作用. 中国药理学报.1986，7(2)：110-113
[4] Sahelian R. Huperzine A [J]. Better nutrition.1999，61(5)：26
