药学本科毕业论文.doc

TQ0701对大鼠脑缺血再灌注损伤的保护作用

目录
摘要 1
前言 3
第一章 材料 6
1.1 药物和试剂 6
1.2 主要仪器 7
1.3 动物 7
第二章 方法 8
2.1 TQ0701iv对局灶性脑缺血大鼠的神经功能和脑组织梗塞率的影响 8
2.2 TQ0701iv对局灶性脑缺血大鼠脑组织学的影响 9
2.3 统计学处理 9
第三章 结果 10
3.1 TQ0701对局灶性脑缺血大鼠的神经功能和脑组织梗塞率的影响 10
3.2 TQ0701对局灶性脑缺血大鼠脑组织学的影响 10
第四章 讨论 12
参考文献 14
致谢 16

摘要：目的: 探讨新型的化合物TQ0701对大鼠脑缺血再灌注损伤保护作用的有效性，从而为该化合物的机制研究以及临床应用开发提供相关实验数据和实验思路。
方法: 将60只雄性SD大鼠随机分为假手术组、模型组、阳性对照组（依达拉奉3mg/kg）、TQ0701低剂量组(1.5mg/kg)、中剂量组(3mg/kg)、高剂量组(6mg/kg)各10只。假手术组仅进行手术而不造成缺血状态，其余各组均采用Longa线栓法制备大鼠MCAO模型，在缺血2h后进行再灌注。给药组和阳性对照组分别在缺血前30min以及再灌注0、2h尾静脉注射TQ0701和依达拉奉，假手术组和模型组则给予等量的0.9%氯化钠溶液。再灌注24h后观察大鼠神经功能评分、脑组织梗塞百分率和病理组织学的改变。
结果: 模型组大鼠的神经功能评分和脑组织梗塞百分率同假手术组、阳性对照组以及TQ0701三个剂量组相比有显著差异，阳性对照组与TQ0701三个剂量组相比未见显著差异。同时电镜观察结果也发现TQ0701三个剂量组及阳性对照组与模型组相比神经细胞病理改变较轻。
结论：化合物TQ0701对急性脑梗死有明显的保护作用。
关键词：TQ0701；脑缺血再灌注；依达拉奉；


Protective effects of TQ0701 on cerebral ischemic reperfusion injury in rats
Abstract: Aim: To investigate the effects of TQ0701 by dynamic changes of infarct volumes and neurological deficit scores on local cerebral ischemic-reperfusion injury in rats. Method: Male SD rats were randomly divided into sham-operated control group (n=10), normal saline control groups (n=10), edaravone-treated group (3mg/kg) (n=10) and TXL-treated groups (6mg/kg, 3mg/kg and 1.5mg/kg) (n=10 each). Animals in the latter four groups were subjected to transient focal ischemia by the middle cerebral artery occlusion (MCAO) for 120 minutes. The rats were pretreated with normal saline or TXL 30min before ischemic and 0min, 2hours after reperfusion. After 24hours of reperfusion, infarct volumes were determined by 2, 3, 5-triphenyltetrazolium chloride (TTC) and neurological scores were investigated. Results: Infarct volumes in TQ0701-treated groups were significantly decreased compared with those in control groups at 24 after MCAO, and the neurological deficit scores in TQ0701-treated groups were synchronously improved. Conclusion: These results show that treatment with TQ0701 can attenuate brain injury and TQ0701 may be a potential neuroprotective agent in cerebral ischemia-reperfusion.
Key words: TQ0701; cerebral ischemic reperfusion; edaravone