生物专业本科毕业论文设计.doc
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生物专业本科毕业论文设计,本文是本人的本科毕业论文，重要内容包括解磷菌株的筛选，以及对其各种不同发酵条件和参数下其解磷能力的研究。内容涉及微生物方面的各种实验、分析和研究方法，详见目录，内容充实，图文并茂，物有所值，欢迎下载目 录1 引言12 材料与方法22.1 主要仪器设备22.2 菌种的分离与纯化22.2.1 分离培养基32.2.2 菌种分...
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本文是本人的本科毕业论文，重要内容包括解磷菌株的筛选，以及对其各种不同发酵条件和参数下其解磷能力的研究。内容涉及微生物方面的各种实验、分析和研究方法，详见目录，内容充实，图文并茂，物有所值，欢迎下载
目 录
1 引言 1
2 材料与方法 2
2.1 主要仪器设备 2
2.2 菌种的分离与纯化 2
2.2.1 分离培养基 3
2.2.2 菌种分离 3
2.2.3 菌种纯化 3
2.3 解磷能力的初步测定 4
2.4 不同条件对菌株的解磷能力的影响 4
2.4.1 测定原理与方法 4
2.4.2 不同培养时间对解磷能力的影响 5
2.4.3 不同碳源对解磷能力的影响 6
2.4.4 不同氮源对解磷能力的影响 6
2.4.5 不同的磷酸钙量对解磷能力的影响 6
2.4.6 不同盐浓度对解磷能力的影响 6
3 结果与分析 7
3.1 分解无机磷菌株的筛选结果 7
3.2 不同条件对解磷能力的影响 8
3.2.1 不同培养时间对菌株解磷能力的影响 8
3.2.2 不同碳源对解磷能力的影响 9
3.2.3 不同氮源对解磷能力的影响 10
3.2.4 不同的磷酸钙量对解磷能力的影响 11
3.2.5 不同盐浓度对解磷能力的影响 12
4 讨论与结论 13
4.1 讨论 13
4.2 结论 14
参考文献 15
致谢 16
附录1相关英文文献 17
附录2英文文献中文译文 24
摘 要
本研究利用无机磷固体培养基，通过划线分离的方法，从人工湿地中采集的样品中筛选出具有分解无机磷能力的菌株共6株，通过比较D/d值初步确定解磷能力的大小，选择D/d值较大的4株，采用不同碳源、不同氮源、不同磷酸钙含量和不同盐浓度等液体培养基，用钼锑抗分光光度法测定各菌株对无机磷的分解能力，从而讨论不同条件对其解磷能力的影响。研究结果表明：4个菌株最容易利用的碳源是葡萄糖，而最不容易利用的碳源是蔗糖，碳源种类对解磷能力影响较大；氮源种类对各菌株的解磷能力影响较小；磷酸钙含量为1%时，4个菌株解磷能力最强，磷酸钙含量为4%时解磷能力受到严重抑制；NaCl浓度为4%时，4个菌株对无机磷分解能力最强；当NaCl浓度达到10%，各菌株的解磷能力受到严重抑制。
关键词：人工湿地；分解无机磷菌株；解磷能力
ABSTRACT
Using solid medium that contained inorganic phosphorus and the method of streak, 6 phosphate-solubilizing bacterial strains were isolated from the samples taken from artificial wetland. Through comparing the phosphate-solubilizing ability preliminarily, four strains that had larger D/d were studied. Using fluid medium that consisted of the different carbon source, nitrogen source, calcium phosphate content and concentration of NaCl, and Molybdenum antimony anti - spectrophotometric method discussed the influence that diffent conditions on phosphate-solubilizing ability. Some conclusions are obtained. firstly, glucose was the most easy to be used, and sucrose as carbon source was the most difficult to be used for the four strains, and carbon source was a major influence factor for phosphate-solubilizing ability. Secondly, nitrogen source hadn’t an obvious effect on phosphate-solubilizing ability for the four strains. Thirdly, when the calcium phosphate concentration was 1%, they had the largest phosphate-solubilizing capacity. When the calcium phosphate concentration was 4%, their phosphate-solubilizing capacity was restrained seriously. Fourthly, when the concentration of NaCl was 4%, they had the largest phosphate-solubilizing capacity. When the concentration of NaCl was 10%, their phosphate-solubilizing capacity was restrained seriously.
Key words: artificial wetland; decomposing inorganic phosphorus bacteria; phosphate-solubilizing ability
目 录
1 引言 1
2 材料与方法 2
2.1 主要仪器设备 2
2.2 菌种的分离与纯化 2
2.2.1 分离培养基 3
2.2.2 菌种分离 3
2.2.3 菌种纯化 3
2.3 解磷能力的初步测定 4
2.4 不同条件对菌株的解磷能力的影响 4
2.4.1 测定原理与方法 4
2.4.2 不同培养时间对解磷能力的影响 5
2.4.3 不同碳源对解磷能力的影响 6
2.4.4 不同氮源对解磷能力的影响 6
2.4.5 不同的磷酸钙量对解磷能力的影响 6
2.4.6 不同盐浓度对解磷能力的影响 6
3 结果与分析 7
3.1 分解无机磷菌株的筛选结果 7
3.2 不同条件对解磷能力的影响 8
3.2.1 不同培养时间对菌株解磷能力的影响 8
3.2.2 不同碳源对解磷能力的影响 9
3.2.3 不同氮源对解磷能力的影响 10
3.2.4 不同的磷酸钙量对解磷能力的影响 11
3.2.5 不同盐浓度对解磷能力的影响 12
4 讨论与结论 13
4.1 讨论 13
4.2 结论 14
参考文献 15
致谢 16
附录1相关英文文献 17
附录2英文文献中文译文 24
摘 要
本研究利用无机磷固体培养基，通过划线分离的方法，从人工湿地中采集的样品中筛选出具有分解无机磷能力的菌株共6株，通过比较D/d值初步确定解磷能力的大小，选择D/d值较大的4株，采用不同碳源、不同氮源、不同磷酸钙含量和不同盐浓度等液体培养基，用钼锑抗分光光度法测定各菌株对无机磷的分解能力，从而讨论不同条件对其解磷能力的影响。研究结果表明：4个菌株最容易利用的碳源是葡萄糖，而最不容易利用的碳源是蔗糖，碳源种类对解磷能力影响较大；氮源种类对各菌株的解磷能力影响较小；磷酸钙含量为1%时，4个菌株解磷能力最强，磷酸钙含量为4%时解磷能力受到严重抑制；NaCl浓度为4%时，4个菌株对无机磷分解能力最强；当NaCl浓度达到10%，各菌株的解磷能力受到严重抑制。
关键词：人工湿地；分解无机磷菌株；解磷能力
ABSTRACT
Using solid medium that contained inorganic phosphorus and the method of streak, 6 phosphate-solubilizing bacterial strains were isolated from the samples taken from artificial wetland. Through comparing the phosphate-solubilizing ability preliminarily, four strains that had larger D/d were studied. Using fluid medium that consisted of the different carbon source, nitrogen source, calcium phosphate content and concentration of NaCl, and Molybdenum antimony anti - spectrophotometric method discussed the influence that diffent conditions on phosphate-solubilizing ability. Some conclusions are obtained. firstly, glucose was the most easy to be used, and sucrose as carbon source was the most difficult to be used for the four strains, and carbon source was a major influence factor for phosphate-solubilizing ability. Secondly, nitrogen source hadn’t an obvious effect on phosphate-solubilizing ability for the four strains. Thirdly, when the calcium phosphate concentration was 1%, they had the largest phosphate-solubilizing capacity. When the calcium phosphate concentration was 4%, their phosphate-solubilizing capacity was restrained seriously. Fourthly, when the concentration of NaCl was 4%, they had the largest phosphate-solubilizing capacity. When the concentration of NaCl was 10%, their phosphate-solubilizing capacity was restrained seriously.
Key words: artificial wetland; decomposing inorganic phosphorus bacteria; phosphate-solubilizing ability
