结石通片中绿原酸的含量测定.doc
约7页DOC格式手机打开展开
结石通片中绿原酸的含量测定,摘要：目的：建立hplc法测定结石通片中绿原酸含量的方法。方法：采用高效液相法，色谱柱为 sinochrom ods-bp(4.6mm ×250mm5μm),以乙腈：0.5%磷酸溶液（8:92）为流动相，检测波长为326nm，流速为1.0ml/min。结果：绿原酸在浓度为0.008125~0....
内容介绍
此文档由会员 一切如愿8 发布
结石通片中绿原酸的含量测定
摘要：目的：建立HPLC法测定结石通片中绿原酸含量的方法。方法：采用高效液相法，色谱柱为 sinochrom ODS-BP(4.6mm ×250mm 5μm),以乙腈：0.5%磷酸溶液（8:92）为流动相，检测波长为326nm，流速为1.0ml/min。结果：绿原酸在浓度为0.008125~0.13mg/ml呈线性关系r=0.9997（n=6),平均回收率为99.46%，RSD=0.82%。结论：本法简便、准确、快速，适用于结石通片中绿原酸的含量测定。
关键词：高效液相法 结石通片 绿原酸 含量测定
Determination of Chlorogenic Acid in Jieshitong pian by HPLC
Abstract:Objective: Establish a High-performance liquid chromatography (HPLC) to determining of Chlorogenic acid in Jieshitong pian.Methods: Using high performance liquid chromatography, the determination was performed on sinochrom ODS-BP(4.6mm ×250mm 5μm) column,mobile phase for acetonitril- e-0.5% phosphoric acid solution(8:92), detected wavelength for 326 nm, velocity of 1.0 mL/min.Results: The linear range of Chlorogenic acid was 0.4024 -3.2192 u g/ml (r = 0.9997 n = 6),the average recovery is 99.46%, and the RSD (n = 9) is 0.82%. Conclusion: The method was proved to be simple,,reproducible and specific, and it’s propitious to used for determining of Chlorogenic acid in Jieshitong pian.
Keywords: HPLC Jieshitong pian Chlorogenic acid Content determination
摘要：目的：建立HPLC法测定结石通片中绿原酸含量的方法。方法：采用高效液相法，色谱柱为 sinochrom ODS-BP(4.6mm ×250mm 5μm),以乙腈：0.5%磷酸溶液（8:92）为流动相，检测波长为326nm，流速为1.0ml/min。结果：绿原酸在浓度为0.008125~0.13mg/ml呈线性关系r=0.9997（n=6),平均回收率为99.46%，RSD=0.82%。结论：本法简便、准确、快速，适用于结石通片中绿原酸的含量测定。
关键词：高效液相法 结石通片 绿原酸 含量测定
Determination of Chlorogenic Acid in Jieshitong pian by HPLC
Abstract:Objective: Establish a High-performance liquid chromatography (HPLC) to determining of Chlorogenic acid in Jieshitong pian.Methods: Using high performance liquid chromatography, the determination was performed on sinochrom ODS-BP(4.6mm ×250mm 5μm) column,mobile phase for acetonitril- e-0.5% phosphoric acid solution(8:92), detected wavelength for 326 nm, velocity of 1.0 mL/min.Results: The linear range of Chlorogenic acid was 0.4024 -3.2192 u g/ml (r = 0.9997 n = 6),the average recovery is 99.46%, and the RSD (n = 9) is 0.82%. Conclusion: The method was proved to be simple,,reproducible and specific, and it’s propitious to used for determining of Chlorogenic acid in Jieshitong pian.
Keywords: HPLC Jieshitong pian Chlorogenic acid Content determination
