胃复春片中柚皮苷和新橙皮苷的含量测定.doc
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胃复春片中柚皮苷和新橙皮苷的含量测定,摘要：目的建立方法。方法高效液相色谱法，agilent eclipse xdb-c18柱（250mm×4.6mm,5μm），流动是乙腈-水（20:80），检测波长是283nm，流速1.0ml╩min,柱温40℃。结果柚皮苷和新橙皮苷分别在0.03～...
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胃复春片中柚皮苷和新橙皮苷的含量测定
摘要：目的 建立胃复春片中柚皮苷和新橙皮苷的含量测定方法。方法 高效液相色谱法，Agilent Eclipse XDB-C18柱（250mm×4.6mm,5μm），流动是乙腈-水（20:80），检测波长是283nm，流速1.0ml∕min,柱温40℃。结果 柚皮苷和新橙皮苷分别在0.03～0.71、0.02～0.42μg 范围内峰面积与进样量呈现出良好的线性关系，R分别为0.9999和0.9995。柚皮苷的加样回收率在97.1%-99.2%的范围内，平均加样回收率柚皮苷为97.91% ，RSD 为0.8%（n=9）；新橙皮苷的加样回收率在94.3%-97.4%的范围内，平均加样回收率新橙皮苷为96.17%,RSD 为1.2%(n=9),表明本方法回收率良好。结论 本实验方法迅速、灵敏、精准度高、操作难度合适，可以适用于胃复春片中柚皮苷和新橙皮苷的含量测定。
关键词：胃复春片 柚皮苷 新橙皮苷 高效液相色谱法
Determination of Weifuchun film Naringin and Neohesperidin
Abstract: Objective To explore a method for simultaneously determining naringin and neohesperidin in Weifuchun piece by HPLC . Methods HPLC ,AgilentEclipseXDB-C18column (250mm × 4.6mm, 5μm), The mobile phase was composed of acetonitrile-water(20:80), The detection wavelength was 283nm, The flow rate was 1.0ml/min, The column temperature was 40 ° C.Results naringin and, neohesperidin respectively, in the range of 0.03 ~0.71,0.02 ~ 0.42μg peak area showed a good linear relationship,the R were 0.9999and0.9995. The naringin recovery in the range of 97.1% -99.2%. The average recoveries rate naringin was 97.91.%, RSD 0.8% (n = 9)；Neohesperidin recoveries in the range of 94.3% -97.4%, the average recovery rate neohesperidin was 96.17%, RSD 1.2% (n = 9)Conclusion the method could be rapid, sensitive, high accuracy, the difficulty of the operation was appropriate in in Weifuchun Tablets naringin and hesperidin Determination。
Keywords: Weifuchun piece Naringin Neohesperidin HPLC
摘要：目的 建立胃复春片中柚皮苷和新橙皮苷的含量测定方法。方法 高效液相色谱法，Agilent Eclipse XDB-C18柱（250mm×4.6mm,5μm），流动是乙腈-水（20:80），检测波长是283nm，流速1.0ml∕min,柱温40℃。结果 柚皮苷和新橙皮苷分别在0.03～0.71、0.02～0.42μg 范围内峰面积与进样量呈现出良好的线性关系，R分别为0.9999和0.9995。柚皮苷的加样回收率在97.1%-99.2%的范围内，平均加样回收率柚皮苷为97.91% ，RSD 为0.8%（n=9）；新橙皮苷的加样回收率在94.3%-97.4%的范围内，平均加样回收率新橙皮苷为96.17%,RSD 为1.2%(n=9),表明本方法回收率良好。结论 本实验方法迅速、灵敏、精准度高、操作难度合适，可以适用于胃复春片中柚皮苷和新橙皮苷的含量测定。
关键词：胃复春片 柚皮苷 新橙皮苷 高效液相色谱法
Determination of Weifuchun film Naringin and Neohesperidin
Abstract: Objective To explore a method for simultaneously determining naringin and neohesperidin in Weifuchun piece by HPLC . Methods HPLC ,AgilentEclipseXDB-C18column (250mm × 4.6mm, 5μm), The mobile phase was composed of acetonitrile-water(20:80), The detection wavelength was 283nm, The flow rate was 1.0ml/min, The column temperature was 40 ° C.Results naringin and, neohesperidin respectively, in the range of 0.03 ~0.71,0.02 ~ 0.42μg peak area showed a good linear relationship,the R were 0.9999and0.9995. The naringin recovery in the range of 97.1% -99.2%. The average recoveries rate naringin was 97.91.%, RSD 0.8% (n = 9)；Neohesperidin recoveries in the range of 94.3% -97.4%, the average recovery rate neohesperidin was 96.17%, RSD 1.2% (n = 9)Conclusion the method could be rapid, sensitive, high accuracy, the difficulty of the operation was appropriate in in Weifuchun Tablets naringin and hesperidin Determination。
Keywords: Weifuchun piece Naringin Neohesperidin HPLC
