冷诱导条件下番茄cdna文库的构建及slnac29的克隆与表达分析.doc
约23页DOC格式手机打开展开
冷诱导条件下番茄cdna文库的构建及slnac29的克隆与表达分析,冷诱导条件下番茄cdna文库的构建及slnac29的克隆与表达分析摘要：低温是限制冷敏感植物产量和地理分布的重要因素。冷害严重影响番茄的产量和品质。因此，研究并提高其抗寒性具有重要的理论和现实意义。本研究将番茄幼苗置于4℃下处理，取其叶片为试材，构建了差减cdna文库，为番茄重要抗寒基因的挖掘奠定了基础。分析冷胁迫下基...
内容介绍
此文档由会员 小花仙66 发布
冷诱导条件下番茄cDNA文库的构建及SlNAC29的克隆与表达分析
摘要：低温是限制冷敏感植物产量和地理分布的重要因素。冷害严重影响番茄的产量和品质。因此，研究并提高其抗寒性具有重要的理论和现实意义。本研究将番茄幼苗置于4℃下处理，取其叶片为试材，构建了差减cDNA文库，为番茄重要抗寒基因的挖掘奠定了基础。分析冷胁迫下基因表达情况，共获得91个差异表达基因。根据文库信息，利用RT-PCR技术从冷处理的番茄叶片中克隆到一个NAC基因。该基因全长为1243 bp，包括828 bp的开放阅读框，编码276 个氨基酸。通过qRT-PCR分析，该基因受低温及高温诱导表达。
关键字：cDNA文库，番茄，SlNAC29，低温
Construction of a tomato cDNA library induced by cold and cloning and expression analysis of SlNAC29
Abstract：Low temperature is the major factor limiting the productivity and geographical distribution of chilling-sensitive plant species. Therefore, it was theoretically and practically significant to study and enhance the cold-tolerance in plants. A subtractive cDNA library of Lycopersicon esculentum Mill was constructed using the 4℃ treated leaves, lying the foundation for selecting and cloning tomato cold- tolerance gene. The expression pattern of cold related gene was analyzed and 91 genes which were differently expressed were obtained. Furthermore, one NAC gene was cloned from the library. The length of this gene was 1243bp, containing a ORF of 828 bp. This gene encoded a protein of 276 amino acids. By analyzing of qRT-PCR, this gene was induced expression by low temperature and high temperature.
Key words：cDNA library, tomato, SlNAC29, low temperature
目录
摘要····································································································1
关键字·································································································1
1前言··································································································2
1.1 cDNA文库····················································································2
1.1.1 cDNA文库的特点··········································································2
1.1.2 cDNA文库构建方法···································································2
1.2 NAC转录因子家族··········································································3
1.2.1 NAC转录因子的起源·································································3
1.2.2 NAC转录因子的结构与分类························································3
1.2.3 NAC转录因子的生物学功能························································5
1.2.3.1影响植物的生长发育·····························································5
1.2.3.2参与多种植物逆境胁迫防御反应··············································5
1.2.4 NAC转录因子的表达调控···························································6
1.3 主要技术路线···············································································6
2 材料与方法························································································6
2.1 试验材料与处理············································································6
2.1.1 植物材料················································································6
2.1.2 材料处理················································································6
2.1.3菌株与载体··············································································7
2.1.4酶及生化试剂································································..
摘要：低温是限制冷敏感植物产量和地理分布的重要因素。冷害严重影响番茄的产量和品质。因此，研究并提高其抗寒性具有重要的理论和现实意义。本研究将番茄幼苗置于4℃下处理，取其叶片为试材，构建了差减cDNA文库，为番茄重要抗寒基因的挖掘奠定了基础。分析冷胁迫下基因表达情况，共获得91个差异表达基因。根据文库信息，利用RT-PCR技术从冷处理的番茄叶片中克隆到一个NAC基因。该基因全长为1243 bp，包括828 bp的开放阅读框，编码276 个氨基酸。通过qRT-PCR分析，该基因受低温及高温诱导表达。
关键字：cDNA文库，番茄，SlNAC29，低温
Construction of a tomato cDNA library induced by cold and cloning and expression analysis of SlNAC29
Abstract：Low temperature is the major factor limiting the productivity and geographical distribution of chilling-sensitive plant species. Therefore, it was theoretically and practically significant to study and enhance the cold-tolerance in plants. A subtractive cDNA library of Lycopersicon esculentum Mill was constructed using the 4℃ treated leaves, lying the foundation for selecting and cloning tomato cold- tolerance gene. The expression pattern of cold related gene was analyzed and 91 genes which were differently expressed were obtained. Furthermore, one NAC gene was cloned from the library. The length of this gene was 1243bp, containing a ORF of 828 bp. This gene encoded a protein of 276 amino acids. By analyzing of qRT-PCR, this gene was induced expression by low temperature and high temperature.
Key words：cDNA library, tomato, SlNAC29, low temperature
目录
摘要····································································································1
关键字·································································································1
1前言··································································································2
1.1 cDNA文库····················································································2
1.1.1 cDNA文库的特点··········································································2
1.1.2 cDNA文库构建方法···································································2
1.2 NAC转录因子家族··········································································3
1.2.1 NAC转录因子的起源·································································3
1.2.2 NAC转录因子的结构与分类························································3
1.2.3 NAC转录因子的生物学功能························································5
1.2.3.1影响植物的生长发育·····························································5
1.2.3.2参与多种植物逆境胁迫防御反应··············································5
1.2.4 NAC转录因子的表达调控···························································6
1.3 主要技术路线···············································································6
2 材料与方法························································································6
2.1 试验材料与处理············································································6
2.1.1 植物材料················································································6
2.1.2 材料处理················································································6
2.1.3菌株与载体··············································································7
2.1.4酶及生化试剂································································..
