毕业论文-两亲性聚酯薄膜表面的制备与表征.doc

摘  要：两亲性共聚物以其优越的性能开始备受瞩目，采用聚对苯二甲酸乙二醇酯(PET)为基材制备出两亲性的薄膜表面具有广泛的开发应用前景。本论文采用分子设计的方法，以聚乙二醇单甲醚(mPEG)和丙烯酰氯为原料，通过酯化反应对mPEG进行末端功能化修饰；在PET薄膜表面引发原子转移自由聚合接枝mPEG衍生物与苯乙烯的共聚物，得到具有两亲性表面的功能化PET薄膜。研究聚合时间以及共聚单体比例对接枝效果及两亲性能的影响。利用衰减全反射傅立叶变换红外光谱仪(FTIR/ATR)、X-射线光电子能谱(XPS)、扫描电子显微镜(SEM)和接触角测试仪(CA)对修饰后聚酯薄膜进行表征。采用大肠杆菌(E.coli)粘附实验考察改性前后PET薄膜表面的微生物的粘附性关系。测试结果表明：成功制备mPEG末端功能化单体，SI-ATRP方法在PET薄膜表面接枝上了两亲性mPEG衍生物与苯乙烯的共聚物层，且随着单体比例的不同共聚物层的组成及性能均发生改变。E.coli粘附实验表明：随着膜表面共聚物层中mPEG含量的增加，E.coli的粘附量减少。

关键字：聚酯薄膜；表面引发原子转移自由基聚合；两亲性；微生物粘附 

Preparation of Polyester Films with Apamphipathilic Surface

Abstract: Amphiphilic copolymer with its superior performance has attracted more and more attention. Using polyethylene terephthalate (PET) as the substrate to prepare film with amphiphilic surface has wide development and application prospect. In this thesis, molecular design methods was adapted, the terminal function of polyethylene glycol monomethyl ether (mPEG) was modified through the way of esterification with polyethylene glycol monomethyl ether and acryloyl chloride as raw materials. And then the copolymer of mPEG derivatives and styrene was grafted from the surface of polyester films through surface initiated atom transfer radical polymerization (SI-ATRP) to prepare functional PET films with amphipathy surface. Research the effects of polymerization time and different feed ratio of mPEG derivatives and styrene on the grafting  effects and amphiphilic properties. The modified PET films were characterized by attenuated total reflection Fourier transform infrared spectroscopy (FTIR/ATR), X-ray photoelectron spectroscopy (XPS) , scanning electron microscopy (SEM) and contact angle(CA). The microbial adhesion of PET film before and after modification was investigated by Escherichia coli (E.coli) adhesion experiment. Test results showed: the terminal function of mPEG was successfully modified; the amphiphilic mPEG derivatives with styrene copolymer layer was grafted onto the surface of the PET film by SI-ATRP , and the composition and properties of the copolymer layer changed with the monomer ratio. The E.coli adhesion experiments showed: As the mPEG content in the copolymer layer on the film surface increases, the adhesion of E.coli reduced.

Key words: polyester films; surface-initiated atom transfer radical polymerization; apamphipathilic; microbial adhesion

目  录

摘  要 I

Abstract II

目  录 III

术 语 表 V

1 绪论 1

1.1 两亲性共聚物发展现状 1

1.2 聚乙二醇单甲醚(mPEG) 1

1.3 聚酯薄膜的现状和发展趋势及其接枝改性的研究现状 1

1.4 两亲性聚酯薄膜在生物医学领域发展趋势 2

1.5 论文研究意义 2

2 实验部分 4

2.1 原料和试剂 4

2.2 主要实验仪器和设备 5

2.3 原料的合成及纯化 5

2.4 PET薄膜表面ATRP引发剂的固定 6

2.5 mPEG衍生物的制备 6

2.6 PET薄膜表面接枝mPEG衍生物和苯乙烯共聚物 7

2.7 细菌粘附实验 7

2.7.1 细菌的复苏、培养 7

2.7.2 细菌生长曲线的测定与比浊法 8

2.7.3 细菌粘附实验操作流程 8

2.8 表征 9

3 结果与讨论 11

3.1 PET薄膜引发剂的X-射线光电子能谱表征 11

3.2 mPEG衍生物的表征 12

3.2.1 mPEG衍生物的红外表征 12

3.2.2 mPEG衍生物的核磁表征 13

3.3 PET薄膜表面接枝两亲性mPEG衍生物与苯乙烯共聚物的表征 13

3.3.1 PET薄膜表面接枝两亲性mPEG衍生物与苯乙烯共聚物的红外表征 13

3.3.2 PET薄膜表面接枝mPEG衍生物/苯乙烯共聚物的接枝量计算与分析 14

3.3.3 PET薄膜表面接枝mPEG衍生物/苯乙烯共聚物的接触角分析 15

3.3.4 PET薄膜表面接枝mPEG衍生物/苯乙烯共聚物的表面自由能的计算及结果分析 18

3.3.5 PET薄膜表面接枝mPEG衍生物/St共聚物的表面形貌 19

3.4 细菌粘附实验结果分析 20

3.4.1 大肠杆菌生长曲线测定分析 20

3.4.2 接枝mPEG衍生物和St共聚物改性前后的PET薄膜大肠杆菌的粘附性状况 21

3.4.3 PET薄膜的表面性能与大肠杆菌的粘附性关系 22

4 结论 24

参 考 文 献 25

致 谢 27