松茸多糖对辐射损伤小鼠免疫功能的防护作用研究.doc

松茸多糖对辐射损伤小鼠免疫功能的防护作用研究

[摘要]目的：观察松茸多糖对辐射损伤小鼠各项免疫指标的影响，探讨松茸多糖对辐射损伤小鼠免疫功能的防护作用。 方法：将ICR小鼠随机分成三组：正常对照组（NC），辐射对照组（IC）及松茸多糖组（PTM）。PTM组按800mg/kg剂量给予小鼠灌胃，每天一次，连续十一天。第七天除NC组小鼠外给予一次性2.0Gy X射线照射。末次灌胃后第二天将小鼠处死，检测白细胞数，脾、胸腺细胞数，脾、胸腺系数，脾细胞对ConA刺激指数（SI）。  结果：IC组与NC组相比，白细胞计数，脾、胸腺细胞计数，脾、胸腺系数，脾细胞刺激指数（SI）均有显著性降低（p<0.05）。PTM组与IC组相比，白细胞计数，脾、胸腺细胞计数，脾、胸腺系数，脾细胞刺激指数（SI）均有显著性增高（p<0.05）。 结论：松茸多糖对辐射损伤小鼠免疫功能具有防护作用。

[关键词]：松茸多糖；辐射损伤；免疫功能

Study on the immunity protective effect of PTM 

in irradiated mice

 (School of Public Health, Jilin University, Changchun, 130021)

[Abstract] Objective: To observe the immunity protective effect of polysaccharide of tricholoma matsutake (PTM) in irradiated mice。 Methods: The ICR mice were randomly divided into three groups：normal control group（NC）,irradiated control group（IC）, polysaccharide of tricholoma matsutake group (PTM) . The PTM group was administered by intragastric administration with a dose of 800mg/kg for 11 d . All groups except NC group were irradiated once with 2.0Gy X-ray on 7th. Immunity parameters including the number of WBC、 spleen 、thymus gland cells, the organ coefficient of spleen、thymus gland and SI were observed on 6th day after irradiation.   Results: The number of WBC、 spleen 、thymus gland cells, the organ coefficient of spleen、thymus gland and SI of PTM group were higher than that in IC group. There was a significant difference between PTM group and IC group(p<0.05).  Conclusion: PTM has the immunity protective effect in irradiated mice.

[Keywords] polysaccharide of tricholoma matsutake（PTM）；radiation breakdown；immunity