毕业论文-紫外诱变选育耐高温的醋酸杆菌的研究.rar

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目录

引言……………………………………………………………………………………1

绪论……………………………………………………………………………………2

1.1醋酸菌的性质及分类……………………………………………………………2

1.2耐高温产酸发酵研究现状…………………………………………………2

1.3醋酸的应用…………………………………………………………………3

1.4菌种筛选及诱变方法………………………………………………………3

1.4.1菌种筛选方法……………………………………………………………3

1.4.2诱变方法…………………………………………………………………4

1.5菌种产物的检测方法……………………………………………………………4

1.6本课题研究的主要内容及意义…………………………………………………4

第二章 实验材料与方法……………………………………………………………6

2.1药品试剂及仪器设备……………………………………………………………6

2.1.1试剂……………………………………………………………………………6

2.1.2仪器设备………………………………………………………………………6

2.2菌种来源…………………………………………………………………………6

2.3培养基……………………………………………………………………………6

2.4菌种筛选…………………………………………………………………………7

2.5菌体生长曲线的测定……………………………………………………………7

2.6诱变选育…………………………………………………………………………7

2.6.1诱变选育………………………………………………………………………7

2.6.2计算菌种致死率………………………………………………………………8

2.7耐热能力实验……………………………………………………………………8

2.8正交优化…………………………………………………………………………8

第三章  结果与分析…………………………………………………………………9

3.1显色剂显色………………………………………………………………………9

3.2培养基显色………………………………………………………………………9

3.3单菌落培养………………………………………………………………………11

3.4摇瓶培养情况……………………………………………………………………12

3.5醋酸菌的显微镜观察……………………………………………………………12

3.6菌体浓度和产酸量的测量………………………………………………………13

3.6.1菌体生长曲线绘制……………………………………………………………13

3.6.2产酸量曲线……………………………………………………………………14

3.7菌种筛选…………………………………………………………………………15

3.7.1筛选初始菌种…………………………………………………………………15

3.7.2筛选诱变菌株…………………………………………………………………16

3.8正交实验…………………………………………………………………………17

3.8.1单因素的影响…………………………………………………………………17

3.8.2三因素的正交实验……………………………………………………………19

3.9筛选耐高温的菌株………………………………………………………………19

3.9.1初步确定所耐最高温度………………………………………………………19

3.9.2验证所耐最高温度……………………………………………………………20

第四章 结论和展望…………………………………………………………………21

4.1结论………………………………………………………………………………21

4.2展望………………………………………………………………………………21

致 谢…………………………………………………………………………………23

参考文献………………………………………………………………………………24

附表A英文参考文献翻译

附表B主要参考文献题录及摘要

摘    要

醋酸是重要的有机酸，在医药、食品、日化各个领路都发挥重要作用。本课题采用的是生物法来获得产醋酸的菌株，探寻获得最大经济效益的方法，因此,加强对醋酸的研究十分必要。本课题具体为研究以酒精为唯一碳源的培养基筛选出产醋酸的菌株。菌种的初筛采用变色圈，产物的检测选用酸碱滴定法。

结合紫外诱变选育提高产酸量，为了筛选出产率高的菌株，下一步需要对菌种的培养条件进行优化，筛选出高产的菌株。并对这些菌株进行菌种鉴定。确定最佳培养条件：PH值为6.0，酒精作为唯一碳源的添加量为3%，接种量为5%。筛选出耐高温的菌株，确定34℃是诱变后菌株所耐最高温度。

本实验所筛选菌种经强化确认后，就可运用到工业生产中；变色圈法对于筛选醋酸杆菌很有帮助；紫外诱变技术简单，但工作量较大。

关键词：醋酸杆菌;菌种筛选;紫外诱变;耐高温

Study on Ultraviolet Mutagenesising and Breeding of High Temperatur Resistant Acetobacter aceti

Abstract

Acetic acid is an important organic acids .It plays avery important roles in medicine，food,daily chemical.In order to obtain the maximum economic efficiency ,what this topic uses is the biological law obtains produces acid. Therefore, it is very essential for strengthen to the acid research. In this research，This topic makes concrete for the research produces the acid take the alcohol as only carbon source culture medium screening the strain. The mold mushroom spawn initially sieves uses the method of color deterioration circle .The product examination selects Acid-base titration method.

Combined with uv mutation breed in improving acid consumption.In order to screen the high production the strain,It needs to carry on the optimization to mold mushroom spawn's raise condition and carry on the mold mushroom spawn appraisal to these strains.Determine the optimum culture conditions as follows:pH value is 6.0,Alcohol as the only carbon source of additives is 3%,quantity is 5%.High temperature resistant strains is screened.34 ℃ is induced by the highest temperature resistant strains after mutagenesis.

the strains of this study should be strengthened before it can be applied to industrial production. the method of color deterioration circle is helpful to screen Acetobacter aceti.The technology of Ultraviolet mutagenesis is simple，but working capacity is very large. 

Key words:Acetobacter aceti;Strains filtration;Ultraviolet mutagenesis;High temperature resistant