毕业设计-年产10000吨高温α-淀粉酶发酵工厂设计.rar

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摘要

    耐高温a一淀粉酶是目前最重要的工业酶制剂之一，约占酶制剂市场25%的比例。耐高温a一淀粉酶能在较高温度下能够有效快速的分解淀粉，被广泛应用于纺织退浆、发酵、制糖等高温条件下生产工业。枯草芽抱杆菌是国内生产耐高温a一淀粉酶的代表菌株之一。

    为了获得较高的酶活和产量，在实验中,对耐高温a一淀粉酶产生菌枯草芽孢杆菌的发酵培养条件进行了优化.综合考虑了所有影响酶活的因素，并对培养条件进行了统计分析，得到产耐高温a一淀粉酶的最佳培养条件。结果表明:优化培养基的组成(ZoomL)为玉米粉25，豆粉25，玉米浆10mL/L，CaCL2 4 Na2HPO4 3,柠檬酸钠 3，PH6.8~7.0.该营养条件下的酶活为2329u’mL一，，约是培养基优化前酶活的12.6倍，酶活有了显著的提高

 

关键词:a一淀粉酶，耐高温，枯草芽抱杆菌.

Annual output of 10000 tons of high temperatureα- amylase fermentation plant design

ABSTRACT

Thermostable a-amysle, catalyzing hydrolysis of starch at high temperatures,is one of the most important .It is widely applied in desinzing,sugar and fermentation industry.bacillus subtilis is one of the industrial strains,which secretes thermostable a-amylase at high level

In the study ,the optimum medium for Bacillus subtilis .On the basis of single factor experiments,the prime factors affecting enzyme activity were selected.And then,the factors were optimized by response surface analysisThe result shows that the optimal medium composition was as follows:Corn flour25,Soybean meal25,Corn syrup 10mL/L, CaCL2 4, Na2HPO4 3,Sodium citrate3,PH6.8~7.0.Enzyme activity under the optimal culture conditions 2329 u/Ml.which is 12.6 fold of the orignai medium,this study has a significant advancement.The experimental data under various have validated the theoretical values

KEY WORDS:a一amylase，thermostable，Bacillus subtilis

目录

引言························································· 1

第一章 绪论·················································  3

1.1 高温α-淀粉酶·············································3

1.2 高温α-淀粉酶的产生菌的生物学特征·························3

1.3 高温α-淀粉酶的国内外研究概况·····························3

1.3.1 高温α-淀粉酶的国外研究概况·····························3

1.3.2 耐高温α-淀粉酶的国内研究·······························4

1.4 高温淀粉酶的应用 ·········································5

1.4.1 纺织物退浆············································· 6

1.4.2 酒精生产··············································· 6

1.4.3 淀粉糖生产··············································6

第2章 生产工艺流程···········································8

2.1 生产方法··················································8

2.2 工艺流程··················································8

2.3 操作工艺··················································8

2.3.1原料的处理··············································8

2.3.2 发酵工序················································8

第3章 发酵车间设备设计与选型·································9

3.1发酵罐的选型···············································9

3.1.1发酵罐容积与台数的确定···································9

3.1.2主要尺寸的计算·········································· 9

3.1.3 发酵罐冷却面积的计算····································10

3.1.4发酵罐搅拌器的设计······································10

3.1.5 发酵罐设备结构的工艺设计································11

3.1.6发酵罐设备材料的选择····································13

3.1.7发酵罐壁厚的计算········································13

3.1.8发酵罐接管设计··········································13

3.1.9发酵罐支座的选择······································· 14

3.2发酵罐的选型··············································14

3.2.1种子罐容积和数量的选择··································14

3.2.2 种子罐主要尺寸的确定····································14

3.2.3 种子罐型号的确定········································15

第四章 工艺计算书·············································16

4.1 物料衡算··················································16

4.1.1工艺技术指标及基础数据···································16

4.1.2 原料消耗计算（基准：一吨成品高温淀粉酶）··················16

4.1.2.1 所需发酵罐数量·········································16

4.1.2.2 总物料衡算·············································17

4.1.3 接种量···················································17

4.1.4 成品高温淀粉酶···········································17

4.2 热量衡算···················································18

4.2.1液化热平衡计算············································18

4.2.2 发酵过程中的蒸汽耗量的计算·······························18

4.2.3 发酵过程中的冷却水耗量计算·······························19

4.2.4 发酵过程中的无菌空气耗用量的计算·························20

第5章  全厂及车间布置·········································21

第六章 “三废”处理············································23

第七章 经济概算················································24

7.1 投资概算···················································24

7.1.1用于建筑工程的各项费用···································24

7.2 利润、投资回收期计算·······································24

7.2.1利润·····················································24

7.2.2 投资回收期···············································24

7.3 小结·······················································24

结论与展望·····················································25

致谢···························································26

参考文献·······················································27