番茄花叶病毒（tomv）的分子鉴定及检测分析.doc

全文1.4万字 16页
摘要：
番茄花叶病毒(Tomato mosaic virus，ToMV)于1909年首次在美国报道，是一类世界性分布的植物病毒, 能在多种作物上引起严重病害, 造成重大经济损失，ToMV的寄主范围很广，番茄是其主要寄主。本文利用分子生物学方法，从引起花叶的加工番茄病样上，检测到ToMV，在分子水平上证实ToMV存在；并且利用ToMV的单克隆抗体， 建立ELISA检测系统，对部分种植加工番茄地区ToMV进行田间检测，以便弄清各加工番茄地区，ToMV的发生情况，从而为加工番茄的生产、抗病育种和防治提供理论依据。

Abstract：
The PCR product of isolate XJ152-1 was cloned and sequenced. Sequence comparisons showed that it consisted of 690 nt, with a 480 nt open reading frame (ORF), had high sequence identities (more than 97%) with other reported ToMV and the ORF encoded CP protein. Field samples were detected by ELISA with monoclonal antibody to ToMV. The results showed that ToMV was distributed in Shihezi, Manas and Yanqi. 13 processing tomato cultivars were also detected by ELISA in field for Evaluation of disease resistance to ToMV. The results demonstrated that none of them was resistant to ToMV and 2 were susceptible.

Key words: processing tomato；molecular identification； serological test


参考文献：
[1]于翠．番茄花叶病毒（ToMV）和黄瓜花叶病毒（CMV）单克隆抗体制备及ToMV在烟草上致病
分子机理研究[D]．浙江大学博士学位论文,2004
[2]冯兰香，蔡少华，郑贵彬等．我国番茄病毒病的主要毒原种类和番茄上烟草花叶病毒株系的鉴定
[J]. 中国农业科学, 1987，20（3）：60-66
[3]李向东，李怀方，范在丰．马铃薯Y病毒属病毒的分子生物学研究进展[J]．微生物学通报，1999,
26（4）283-285