薇甘菊种群遗传多样性的issr分析.doc
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薇甘菊种群遗传多样性的issr分析,薇甘菊种群遗传多样性的issr分析页数：14字数：8908摘要：采用issr技术对广东省深圳市周围8个薇甘菊自然种群的遗传多样性进行分析。3个引物共检测到37个位点，其中多态位点30个，多态位点百分率81.08%。物种水平的shannon信息指数为0.4172，nei指数为0.2808，表明物种水平的遗传多样性很高；种...
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薇甘菊种群遗传多样性的ISSR分析
页数：14 字数：8908
摘 要：采用ISSR技术对广东省深圳市周围8个薇甘菊自然种群的遗传多样性进行分析。3个引物共检测到37个位点，其中多态位点30个，多态位点百分率81.08%。物种水平的Shannon信息指数为0.4172，Nei指数为0.2808，表明物种水平的遗传多样性很高；种群水平的遗传多样性也较高，多态位点百分率平均为57.43%，Shannon信息指数平均为0.3613,Nei指数平均为0.2521。AMOVA分子变异显示4.41% 的变异存在于种群间，95.59%的变异存在于种群内。种群间的基因流较高，为4.3946，遗传相似度平均为0.9564，遗传距离平均为0.0448。聚类分析显示，8个薇甘菊自然种群的遗传距离与地理距离存在一定的相关性。
关键词：薇甘菊；自然种群；遗传多样性；遗传分化；ISSR
Genetic Diversity in the Populations of Mikania micrantha Revealed by ISSR Molecular Markers
Abstract: Genetic diversity in the eight populations of Mikania micrantha around Shenzhen city, Guangdong province were analyzed using inter simple sequence repeat (ISSR) molecular markers.A total of 37 loci, 30 of which were polymorphic loci, the percent of polymorphic loci was 81.08% in the species, while Shannon，s information index and Nei，s gene diversity were 0.4172 and 0.2808, respectively. These indicated that the genetic diversity of Mikania micrantha was relatively high. The mean value of the percent of polymorphic loci, Shannon，s information index and Nei，s gene diversity were 57.43%, 0.3613 and 0.2521, respectively. Analysis of molecular variance (AMOVA) demonstrated that the among-population component accounted for 4.41% of the total variations, while the within-population component accounts for 95.59%. The gene flow was high (4.3946), the genetic similarity and genetic distance with an average of 0.9564 and 0.0448, respectively. The clustering analysis showed that there is a certain correlation between genetic distance and geographic distance of eight natural populations of Mikania micrantha .
Key words: Mikania micrantha；population；genetic diversity；genetic differentiation；ISSR
页数：14 字数：8908
摘 要：采用ISSR技术对广东省深圳市周围8个薇甘菊自然种群的遗传多样性进行分析。3个引物共检测到37个位点，其中多态位点30个，多态位点百分率81.08%。物种水平的Shannon信息指数为0.4172，Nei指数为0.2808，表明物种水平的遗传多样性很高；种群水平的遗传多样性也较高，多态位点百分率平均为57.43%，Shannon信息指数平均为0.3613,Nei指数平均为0.2521。AMOVA分子变异显示4.41% 的变异存在于种群间，95.59%的变异存在于种群内。种群间的基因流较高，为4.3946，遗传相似度平均为0.9564，遗传距离平均为0.0448。聚类分析显示，8个薇甘菊自然种群的遗传距离与地理距离存在一定的相关性。
关键词：薇甘菊；自然种群；遗传多样性；遗传分化；ISSR
Genetic Diversity in the Populations of Mikania micrantha Revealed by ISSR Molecular Markers
Abstract: Genetic diversity in the eight populations of Mikania micrantha around Shenzhen city, Guangdong province were analyzed using inter simple sequence repeat (ISSR) molecular markers.A total of 37 loci, 30 of which were polymorphic loci, the percent of polymorphic loci was 81.08% in the species, while Shannon，s information index and Nei，s gene diversity were 0.4172 and 0.2808, respectively. These indicated that the genetic diversity of Mikania micrantha was relatively high. The mean value of the percent of polymorphic loci, Shannon，s information index and Nei，s gene diversity were 57.43%, 0.3613 and 0.2521, respectively. Analysis of molecular variance (AMOVA) demonstrated that the among-population component accounted for 4.41% of the total variations, while the within-population component accounts for 95.59%. The gene flow was high (4.3946), the genetic similarity and genetic distance with an average of 0.9564 and 0.0448, respectively. The clustering analysis showed that there is a certain correlation between genetic distance and geographic distance of eight natural populations of Mikania micrantha .
Key words: Mikania micrantha；population；genetic diversity；genetic differentiation；ISSR
