bt抗虫棉的研究.doc
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bt抗虫棉的研究,bt抗虫棉的研究.doc页数：18字数：7636摘要为检测bt抗虫棉棉籽及棉籽油对哺乳动物的诱变活性，以评价转bt基因抗虫棉的安全性，本实验以环磷酰胺作为阳性诱变剂，以普通棉的棉籽及棉籽油作为对照物，同时设阴性对照组，以成年封闭群昆明种雄性小鼠为实验材料，将棉籽粉或棉籽油掺入饲料中饲喂小鼠，检测小鼠骨髓嗜多染红细胞（p...
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Bt抗虫棉的研究.doc
页数：18 字数：7636
摘要
为检测Bt抗虫棉棉籽及棉籽油对哺乳动物的诱变活性，以评价转Bt基因抗虫棉的安全性，本实验以环磷酰胺作为阳性诱变剂，以普通棉的棉籽及棉籽油作为对照物，同时设阴性对照组，以成年封闭群昆明种雄性小鼠为实验材料，将棉籽粉或棉籽油掺入饲料中饲喂小鼠，检测小鼠骨髓嗜多染红细胞（Polychromatic erythrocytes , PCE）微核率（Micronucleus frequency, MN(）和精子畸变率（Sperm malformation rate）。实验结果显示，饲喂棉籽及棉籽油饲料的实验组的微核率与阴性对照组相比，大多数P>0.05，无显著性差异，有四组P<0.05，但它们的微核率比阴性对照组小，即这四组的微核率显著小于阴性对照组；与阳性对照组相比，P<0.001，都有极显著差异。上述实验组的精子畸变率与阴性对照组相比，P>0.05，无显著性差异；与阳性对照组相比，P<0.05，有显著性差异。结果表明：在本实验条件下，Bt抗虫棉与普通棉的棉籽及棉籽油对小鼠体细胞和性细胞均未显示出诱变活性，且没有剂量效应。
关键词：Bt抗虫棉, 棉籽, 棉籽油, 嗜多染红细胞微核率, 精子畸变率
Study on Mutagenicity of Cotton Seed and Cotton Seed Oil
of Bt-transgenic Cottons
Abstract
To evaluate the safety of insect resistant cotton plants,which express a Bacillus thuringiensis protein,the mutagenicity of cotton seed and cotton seed oil on mammals was examined.The raw cotton seed and oil was mixed into mice’ diet. Using cyclophosphamide as positive control,non-modified cotton seed and cotton seed oil as controls, this study tested the micronucleus frequency in mice bone marrow polychromatic erythrocytes and the sperm malformation rate.The results showed that there’s no significant difference between most of the seed groups,oil groups and the negative control(P>0.05) or that the micronucleus frequency of the seed and oil groups is significantly lower than the negative control(P<0.05).On the other hand,there existed significant difference between the seed groups,oil groups and the positive control (micronucleus test P<0.001,sperm malformation test P<0.05).
To conclude,under the condition of this test,the seed and seed oil of Bt and non-modified cottons showed no cytomutagenicity or genomutagenicity on mice,and dose effect was not observed.
Key Words: Bt-transg
页数：18 字数：7636
摘要
为检测Bt抗虫棉棉籽及棉籽油对哺乳动物的诱变活性，以评价转Bt基因抗虫棉的安全性，本实验以环磷酰胺作为阳性诱变剂，以普通棉的棉籽及棉籽油作为对照物，同时设阴性对照组，以成年封闭群昆明种雄性小鼠为实验材料，将棉籽粉或棉籽油掺入饲料中饲喂小鼠，检测小鼠骨髓嗜多染红细胞（Polychromatic erythrocytes , PCE）微核率（Micronucleus frequency, MN(）和精子畸变率（Sperm malformation rate）。实验结果显示，饲喂棉籽及棉籽油饲料的实验组的微核率与阴性对照组相比，大多数P>0.05，无显著性差异，有四组P<0.05，但它们的微核率比阴性对照组小，即这四组的微核率显著小于阴性对照组；与阳性对照组相比，P<0.001，都有极显著差异。上述实验组的精子畸变率与阴性对照组相比，P>0.05，无显著性差异；与阳性对照组相比，P<0.05，有显著性差异。结果表明：在本实验条件下，Bt抗虫棉与普通棉的棉籽及棉籽油对小鼠体细胞和性细胞均未显示出诱变活性，且没有剂量效应。
关键词：Bt抗虫棉, 棉籽, 棉籽油, 嗜多染红细胞微核率, 精子畸变率
Study on Mutagenicity of Cotton Seed and Cotton Seed Oil
of Bt-transgenic Cottons
Abstract
To evaluate the safety of insect resistant cotton plants,which express a Bacillus thuringiensis protein,the mutagenicity of cotton seed and cotton seed oil on mammals was examined.The raw cotton seed and oil was mixed into mice’ diet. Using cyclophosphamide as positive control,non-modified cotton seed and cotton seed oil as controls, this study tested the micronucleus frequency in mice bone marrow polychromatic erythrocytes and the sperm malformation rate.The results showed that there’s no significant difference between most of the seed groups,oil groups and the negative control(P>0.05) or that the micronucleus frequency of the seed and oil groups is significantly lower than the negative control(P<0.05).On the other hand,there existed significant difference between the seed groups,oil groups and the positive control (micronucleus test P<0.001,sperm malformation test P<0.05).
To conclude,under the condition of this test,the seed and seed oil of Bt and non-modified cottons showed no cytomutagenicity or genomutagenicity on mice,and dose effect was not observed.
Key Words: Bt-transg
