菊花嫩茎快繁技术的研究.doc

菊花嫩茎快繁技术的研究

摘要：先实验前的准备工作，然后选取菊花，以带顶芽的嫩茎为外植体接种到诱导培养基中，放在培养室里培养并观察，一周后长出丛生芽，再没有污染的丛生芽在无菌超净工作台上用手术刀切成多块，再将丛生芽接到继代培养基中进行继代培养，当发现初步长出嫩根时，将无污染的带根的丛生芽接到生根培养基中，当生长成幼苗时将生根试管苗开盖练苗,移栽。及在培养过程中遇到的问题。
关键词：菊花；组织培养；褐变；脱毒；外植体

Rapid Propagation of Chrysanthemum tender stem Research
Abstract: Tests before first the preparatory work, then the selection chrysanthemum, plants the body take the belt terminal bud tender stem as outside to vaccinate in the induction culture medium, places in the raise room to raise and to observe, after a week is long grows thickly the bud, then does not have the pollution to grow thickly the bud to sliver the multi-blocks on the asepsis ultra only work table with the scalpel, then will grow thickly the bud grafting to continue in a generation of culture medium to carry on continues a generation of raise, when discovered when at the beginning of the length of stride will leave the tender root, will not have the pollution the belt root to grow thickly the bud grafting to take root in the culture medium, when will grow the seedling will take root the test tube seedling to uncap practices the seedling, will transplant. And question which meets in the raise process.
Keyword：Chrysanthemum; Tissue culture; Turning brown; Escapes the poison; Outside plants the body

目      录
1．材料和方法………………………………………………………………8
1．1材料与用具……………………………………………………………8
1．2培养条件……………………………………………………………… 9
1．3实验前的准备工作…………………………………………………… 9
1．3．1培养基配制………………………………………………………… 9
1．3．2接种室的灭菌……………………………………………………… 9
1．3．3超净工作台及器械消毒…………………………………………… 9
1．3．4材料选取…………………………………………………………… 9
1．3．5材料灭菌…………………………………………………………… 9
1．4接种…………………………………………………………………… 10
1．5诱导侧芽生长……………………………………………………………10
1．6 继代培养……………………………………………………………… 10
1．7　诱导生根………………………………………………………………10
1．8　移栽培养………………………………………………………………10
2结果与分析…………………………………………………………………10
2.1　初代培养………………………………………………… 10
2.2　继代培养…………………………………………  11
2．3生根诱导及移栽…………………………………………………………11
2． 4细菌、真菌污染及防治………………………………………………………12
3.讨论……………………………………………………………………… 12
参考文献…………………………………………………………………… 13
致谢………………………………………………………14